克隆

This advanced yet simple solution will help you in hard drive cloning, mass installation and File Backup.

这种先进而简单的解决方案将帮助硬盘克隆，大规模安装和文件备份你。

The expression of MDK was detected by real-time PCR and Western Blot. Results: The retroviral vector pLXSN-MDK was successfully constructed and infected SGC7901 cells,and the MDK gene expression of SGC7901/pLXSN-MDK was up-regulated.

病毒上清感染SGC7901细胞，G418筛选出阳性克隆，经实时定量PCR及Western Blot检测发现SGC7901细胞中MDK mRNA及蛋白表达水平明显上调。

His descendent, Tion Medon, was the Port Administrator of Pau City during the Clone Wars.

他的后代提恩。梅顿在克隆战争时期担任帕城的港口行政长官。

Medon spread the word to the Utapaun resistance, so that when the Republic clone troopers did storm Utapau, they were bolstered by native Utapaun forces.

梅顿向尤塔帕的抵抗组织散布了这一消息。因此当共和国的克隆大军开始冲锋时，他们可以得到本地的武装军队的支援。

Phylogenetic amalysis of the long terminal repeat and env gene of HTLV-I gave a phylogenetic classification of HTLV-I genotypes into five major molecular subtypes~ Cosmopolitan, Japanese , West African , Central African , and Melanesian.

为了解我国HTLV-Ⅰ流行区毒株的主要基因型别，本研究从福建莆田地区克隆出6株HTLV-Ⅰ的env基因和一株tax基因。

We have made good winning on the allograft of melanocyte to vi-tiligo after several years study.

对稳定期白癜风，我们近年开展的异体黑素细胞移植研究已获得突破，该技术距离临床大量使用为期不远；对于活动期白癜风的治疗，仍具有很大的挑战性，我们期望通过清除患者病理性淋巴细胞克隆的研究应对这一挑战。

Objective: To clone whole merozoite surface protein-2 (MSP2) gene of Plasmodiutn falciparum and express it in E.

目的：克隆恶性疟原虫裂殖子表面蛋白2全基因，并在大肠杆菌中进行表达。

Two fragment of Etmic-2 was cloned and sequenced by PCR using genomic DNA as template from sporulated oocysts and the second generation merozoite of Eimeria tenella.

本文利用PCR技术，分别从柔嫩艾美耳球虫的第二代裂殖子和孢子化卵囊基因组DNA中扩增出微线蛋白EtMIC-2基因序列，并进行了克隆和核苷酸序列测定。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐