克隆

Villosa had lower genetic variation (He=0.104) and lower clonal diversity (D=0.764) than other similar plants.

结果表明，和同类植物相比，沙鞭的遗传变异水平和克隆多样性均较低。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

The details of cloning, expression and function of biotinylated luciferase will be discussed in this paper.

本文将就生物素化荧光素酶的克隆、表达以及功能检测进行具体讨论。

Its cDNA had been cloned by RT-PCR. The results of comparison on sequence homology suggested that Luffin P1 may be derived from a vicilin like precursor which is a storage protein in the seeds of Luffa cylindrica.

本文利用3'RACE法克隆了它的cDNA，并构建了它的融合表达质粒pGEX-4T-S2，该质粒在大肠杆菌中获得了成功表达。

BACKGROUND: Otherwise healthy persons with a small number of B-cell clones circulating in the peripheral blood have been designated as having monoclonal B-cell lymphocytosis.

背景：健康人外周血循环中的少量B细胞克隆从另一方面而言被认为是单克隆B细胞性淋巴细胞增多。

To prove that the cloned DNA fragment can express tryptopanase,a new plasmid pET28C-TnaA , in which the cloned DNA fragment was located downstream of T7 promoter on pET28c was constructed and transformed into host BL21(DE3),a BL21 lysogen of bacteriophage DE3 in which the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacuvS promoter ,which is inducible by IPTG.

为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个DNA片段克隆到PET28c质粒的BamHI和HindⅢ位点上，使该片段受T7 RNA聚合酶的启动子控制，然后转化噬菌体DE3的溶源菌BL21(DE3)。

The Cre gene was cloned from lysogen BM25.8 by PCR. The Cre gene was then inserted in between the CaMV35S-35S double promtor and Nos terminator of PBI525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring expression vector pBINPLUSCre.

PCR方法从溶原菌BM25.8基因组DNA扩增出Cre基因，克隆测序验证无误后插入PBI525 CaMV35S-35S双启动子和Nos终止子之间，再将表达盒切下插入pBINPLUS质粒相应位点，得到恢复基因植物表达载体pBINPLUSCre。

We found that PTA1 mAb could upregulate the cytotoxicity of both these two kind of cells.

所得到的一株NK细胞克隆上，PTAI的表达率为61.20。

In this study, a novel EoRab gene was cloned from Euplotes octocarinatus by degenerated primer polymerase chain reaction, with a length of 814 bp in macronucleus and 669 bp in the coding region.

本研究运用PCR技术从原生动物八肋游仆虫大核基因组中克隆获得了一个新的EoRab基因，该基因在八肋游仆虫大核基因组中全长814bp，编码区为669bp，拟编码222个氨基酸。

The mammalia vector of the human OPG can be successfully constructed by gene-engineering, which established the foundation for expressing OPG efficiently and stablily.

采用基因克隆技术成功构建了有表达OPG功能的哺乳类表达载体；此为高效、稳定表达OPG的研究奠定了基础。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐