克隆

The liqase was positively controlled by HAI and AI-2 to some extent. The lecithinase was negatively controlled by HAI-1, and the hemolysin was negatively controlled by both HAI-1 and AI-2. Special primers of luxS gene were designed from the luxS gene sequence of V. harveyi obtained from the GenBank, and the gene was amplified from a pathogenic strain V. harveyi VIB645 by PCR. The luxS gene was cloned to a cloning vector pUC, and was sequenced. It was found that the open reading frame is 519 bp. The similarity of the luxS gene from V. harveyi VIB645 to the published sequence of V. harveyi strain in GenBank was only 90%.

从GenBank中获取哈维氏弧菌中编码AI-2合成酶的luxS基因序列设计一对特异性引物从哈维氏弧菌VIB645中PCR扩增luxS基因，将luxS基因克隆于pUC载体并测序，发现其与GenBank中已发表的哈维氏弧菌的luxS基因序列相似性为90%，证明LuxS/AI-2系统在哈维氏弧菌不同的菌株中的具有一定的特异性，推测LuxS/AI-2系统可能与哈维氏弧菌的致病性或发光现象有关。

WORD PROCESSOR: allows full control of fonts, background+highlight color, left/right/center/justify/indent text, left/right margins, bullets, hyperlinks, table color/shading/border/resize, text-sort, line-select, zoom, 100-level undo/redo, etc. IMAGES: clone, copy, paste, import, export, resize, compress.

文字处理器：允许完全控制的字体，背景+高亮颜色，左/右/中心/理由/缩进文字，左/右的利润率，子弹，超文本链接，表颜色/阴影/边境/大小，文本排序，路线选择，缩放， 100级别的撤销/重做等图像：克隆，复制，粘贴，进口，出口，调整，压缩。

WORD PROCESSOR: allows full control of fonts, background color, highlight color, left/right/center/justify/indent text, left/right margins, bullets, hyperlinks, table color/shading/border/resize, text-sort, line-select, zoom, 100-level undo/redo, line numbers, etc. IMAGES: clone, copy, paste, import, export, resize, compress.

文字处理器：允许完全控制的字体，背景颜色，高亮颜色，左/右/中心/理由/缩进文字，左/右的利润率，子弹，超文本链接，表颜色/阴影/边境/大小，文本排序，线选择，缩放， 100级别的撤销/重做，行号等图像：克隆，复制，粘贴，进口，出口，调整，压缩。

Methods Specific SSU rDNA fragments from nuclear DNA of five Leishmania species and isolates were amplified by PCR. The amplified DNA fragments were cloned into pGEM R-T Easy vector.

nDNA进行PCR扩增，将扩增出的SSUrDNA基因的特异片段克隆于 pGEMR TEasyVector上，采用通用引物M 13进行PCR扩增，全自动测序仪测序。

The vip3A genes amplified from wild-type Bt strains S184 and 611 with different levels of activity against lepidopteran insect larvae were cloned into pGEM-T Easy vector. Alignment of these 2 putative Vip3A proteins with 8 others showed that vip3A genes are highly conservative.

从以上菌株中挑选2个对夜蛾科害虫具有较高和较低毒力的菌株，即S184和611，分别进行vip3A基因的克隆和测序，再已报道的其它8个vip3A基因进行核苷酸和氨基酸序列比较，结果表明，vip3A是一个极其保守的基因。

Bacillus thuringiensis; cry gene; gene cloning; Lepidopteran pest

苏云金芽孢杆菌； cry基因；基因克隆；鳞翅目害虫

With the development of gene cloning technology, ganulocyte colony-stimulating factor recombinant products have been widely used in clinic to provide effective means for the treatment of leucopenia,In recent years, we find the therapy of chemotherapeutics combining with granulocyte colony-stimulating factor is effective in refractory/relapsed/elderly cases of acute leukaemia.

随着基因克隆技术的发展，粒细胞集落刺激因子重组产品已广泛应用于临床，为血液系统疾病的治疗提供了有力手段。目前在临床上广泛用于白细胞减少性疾病。近年来在化疗基础上联用粒细胞集落刺激因子的预激方案也取得良好疗效，为难治、复发、老年急性髓系白血病的治疗创造了机会。

The LIC strategy is efficient for high-throughput cloning and expression, as well as generating of native proteins.

结果表明LIC策略对于高通量克隆和表达是高效的，对于天然蛋白的产生也同样高效。

A new method for construction of a cloning vector for direct ligation with PCR products was described.

本文描述一种构建与PCR产物直接连接的克隆载体的方法。

It is the first time for the segment of F3′H gene to be cloned from the gDNA of ligneous plant.

本研究结果可为梅花类黄酮3′-羟化酶基因全长的克隆奠定基础。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐