克隆

In this paper, the ability and mechanism of antibacterial and immune defense ofChinese shrimp was studied using cytological, biochemical, molecular biological andproteomic methods. A sensitive and stable antibacterial and lysozyme assay methodswere selected. The effect of Vibrio anguillarum and laminarin (a kind of beta-1,3-glucan) on the immune ability of haemolymph of Chinese shrimp was analyzed.The changes of total haemocyte counts, antibacterial ability, lysozyme activity andlysosome membrane stability were detected.

本文采用细胞学，生物化学，分子生物学和蛋白质组学等技术，对对虾的抗菌能力和免疫防御机制进行研究，筛选了灵敏稳定的抑菌和溶菌检测方法；并对灭活鳗弧菌和海带多糖(laminarin，一种B-1， 3 葡聚糖)免疫诱导后的中国明对虾血细胞数目，血淋巴的抑菌活力，溶菌能力，溶酶体膜的稳定性进行分析；对两种重要免疫因子溶菌酶和精氨酸激酶进行克隆和表达分析。

The invention belongs to the biological technical field, in particular to lamprey uncoupling protein molecular clone.

本发明属于生物技术领域。具体涉及七鳃鳗解偶联蛋白(uncoupling protein，UCP)分子克隆。

Luo Lande says, he saw Mooney " clone Twitter " website, his person writes all code, technical level makes a person impressive. On April 18, when interviewing, also admit what Mooney is accepting network of news of Broadcasting Corporation of American whole nation, he is to be in claim to inbreak to the network after having responsibility, just two companies that include ExqSoft inside offer working opportunity actively for him.

罗兰德说，他看了穆尼"克隆Twitter"的网站，所有代码都是他一个人编写的，技术水平令人印象深刻。4月18日，穆尼在接受美国全国广播公司新闻网的采访时也承认，他是在声称对网络入侵负有责任后，才有包括ExqSoft在内的两家公司主动为他提供工作机会的。

The thymidine kinase gene of infectious laryngotracheitis virus Beijing E2 strain was amplified by PCR, cloned into pGEM-T vector, and sequenced. Sequence analyses showed that the TK gene was very closed among sequenced ILTV strains.

以纯化的传染性喉气管炎病毒北京E2株基因组DNA为模板，通过PCR方法扩增了ILTV北京E2株的完整TK基因，将其克隆到pGEM-T载体上，并测定了序列，与其它ILTV毒株TK基因比较说明，ILTV不同毒株的TK基因高度保守。

The genes gB,gC and gD amplified from infectious laryngotracheitis virus Chinese Wanggang strain were cloned into the Eco RⅠ site of the eukaryotic expression vector pCAGGS,re-spectively. The positive recombinant plasmids were screened by transforming into E.coli DH5 α cells,which were named pCAGG-gB,pCAGG-gC and pCAGG-gD respectively after identification by restriction endonuclease analysis and sequencing analysis.

将传染性喉气管炎病毒王岗株的gB、gC、gD基因分别克隆到真核表达载体pCAGGS的 Eco RⅠ位点，经过酶切、测序分析，筛选鉴定出含有gB、gC、gD基因的重组质粒，分别命名为pCAGG-gB、pCAGG-gC、pCAGG-gD。

The genomic DNA A of squash leaf curl virus from Chinahas been cloned and its complete nucleotide sequence was determined.The DNA A consisted of 2441 nucleotides.

对引起我国南瓜曲叶病的病毒分离物DNA的克隆和序列分析表明，中国南瓜曲叶病毒DNA A由2741个核苷酸组成。

The results from DNA sequencing and ELISA showed that tomato yellow leaf curl virus from Senegal and PR China is different new whitefly-transmitted geminivirus separately.

克隆和部分序列分析表明中国和非洲塞内加尔的番茄黄化曲叶病毒分别为不同的粉虱传双生病毒，且均为新的双生病毒。

The AC2 gene fragment of cotton leaf curl virus was obtained from total DNA of CLCuV infected tobacco leaves by polymerase chain reaction, and the amplified DNA fragment was cloned into vector.

以棉花曲叶病毒侵染的烟草叶片组织总DNA为模板，通过聚合酶链反应扩增CLCuV的AC2基因片段并插入克隆载体。

The monomer and dimer clones of full-length genome of cotton leaf curl gemini-virus from Pakistan were constructed , and it was shown that CLCuV-PK could be a geminivirus with monopartite genome.

本研究完成了巴基斯坦棉花曲叶病毒全长基因组单体和双体DNA克隆，证明了CLCuV-PK是一个单组分双生病毒。

It was demonstrated that the cloned plcB gene appeared to be 834 bp in length, entirely identical with that published in GenBank The fusion protein GST-tagged broad- range phospholipase C showed bioactivity and immunogenicity in lecithinase test and Western blotting assay respectively.

结果 克隆的plcB基因长834bp，与GenBank上公布的序列完全相同；表达的与GST标签蛋白融合的广谱磷脂酶C蛋白具有磷脂酶生物活性和免疫原性。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。