克隆

Methods The IPSE gene of S. man soni was synthesized by overlapping PCR, and confirmed by DNA sequencing. The recombinant plasmid IPSE-pET32a was constructed by inserting the gene of IPSE into expression vector pET32a at the downstream of thioredoxin coding sequence.

应用重叠PCR方法合成曼氏血吸虫IPSE基因，将其定向克隆至表达载体pET32a的硫氧还蛋白序列下游，构建重组表达质粒IPSE/pET32a。

Objective:To investigate the virulence,drug resistance and disinfectant resistance of Staphylococcus aureus isolated from surface of substance in medical stations.

目的：了解医疗站物表分离的金黄色葡萄球菌毒力、耐药性及消毒剂抗性，找出优势克隆株，为控制由金黄色葡萄球菌引起的交叉感染提供依据。

Since isolated and purified from Jack bean in 1919, concanavalin A has been used widely especially as an polyclonal mitogen. ConA can stimulate T lymphocytes blast, induce protein-tyrosine phosphorylation in human neutrophils and increase resistance of mice to peritoneal infection by Serratia marcescens.

ConA从1919年被分离纯化以来得到了广泛的应用，是一种有效的多克隆有丝分裂原，可刺激T淋巴细胞增殖及中性粒细胞酪氨酸磷酸化，增强小鼠对白色念珠菌的吞噬功能、对粘质沙雷菌的抵抗力。

Very addictive Jawbreaker clone for Windows, will keep you entertained for a long time.

非常令人上瘾的jawbreaker克隆适用於Windows ，将随时向你受理相当长的时间。

Johnston: I do not think every place will require the development of cloning Xujiahui.

约翰斯顿：我不认为每一个地方的发展将需要克隆徐家汇。

The cattle BMPRⅠA gene full-long cDNA is 4067bp, which encodes 532 amino acids and shares 91%, 93%, 89%, 88%, 91% and 82% similarity in nucleic acid sequences and 97%, 95%, 96%, 95%, 97% and 91% similarity in amino acid sequences with human, chimpanzee, mouse, rat, dog and red jungle fowl, respectively.

克隆得到了牛BMPRⅠA基因4067bp的cDNA全长序列，通过核酸序列分析发现，该基因编码532个氨基酸，与人、黑猩猩、小鼠、大鼠、犬和红原鸡在核酸序列上分别有91%，93%，89%，88%，91%和82%的同源性；在氨基酸序列上分别有97%，95%，96%，95%，97%和91%的同源性。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

He arranged his students in a line. Mahui was to boost the heat of the existing stone while Kagu took on the responsibility of splitting it into two separate spheres. Gibil would then psychokinetically move one of the cloned fireballs to Chantico, who would weave the fire stone incantation. Ikez, Teteo and Gabija would do the same. Nanni's pyrokinetic potential was not as great as the others', but he compensated in arcane intuition.

他让学生们站成一排，玛恚负责给做好的火焰石加热，卡古负责将一个火焰石分成两个不同的火球，吉泊勒运用心灵力量将其中克隆的那块火焰石运送到查安逖克面前，由他给这块火焰石下咒语，艾柯资、特脱和高比佳和查安逖克做同样的工作，兰妮的魔法力比其他人要弱小些，但他的神秘直觉给了他很大的补偿。

Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。