克隆

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

Following confirmation by RFLP and partial sequence analysis, the aliquot mutant viruses (3μg) were used to transfect CEM×174 cell line, and the concentration of SIVmac main core protein(P27) in culture supernatants was closely monitored by enzyme-linked immunosorbent assay in order to identify the replicating ability of the mutants.

一般认为，外膜糖蛋白在病毒的感染和免疫中起重要作用，而SIVmac239和SIVmac142克隆之间envGP区核苷酸的差别，是否提示SIVmac239克隆的致病性或毒力与envGP全区、部分或个别核苷酸有关，仍需要进一步研究〔2，3〕。

Allelism tests revealed that these phenotype-similar mutants could be divided into two complementary groups that were named ll1 and ll2 , respectively.

用图位克隆的方法，克隆了影响拟南芥叶极性发育的重要基因AS1，基因序列分析表明，AS1属于MYB基因家族，可能是一种转录调控因子。

Please make sure you understand the Cloneable concept and the Clone Allocator concept.

请确认你已经理解了可克隆概念和克隆分配器概念。

A new mannose-binding agglutinin gene was cloned from bulbs of Amaryllis vittata Ait. The full-length cDNA of A. vittata agglutinin was 686 bp. The start codon of ava cDNA was at 41-43 bp and the stop codon was at 515-517 bp.

运用同源克隆的方法设计简并引物，通过3'和5'RACE技术，从石蒜科植物朱顶兰总RNA中克隆了编码此凝集素的全长cDNA序列。

A new mannose-binding agglutinin gene was cloned from bulbs of Amaryllis vittata Ait. The fulllength cDNA of A. vittata agglutinin was 686 bp. The start codon of ava cDNA was at 41-43 bp and the stop codon was at 515-517 bp.

运用同源克隆的方法设计简并引物，通过3′和5′RACE技术，从石蒜科植物朱顶兰总RNA中克隆了编码此凝集素的全长cDNA序列。

CDNA fragments of mnh6 and mtp1 genes were cloned from M. grisea appressorium cDNA library and full length DNA fragments of these genes were cloned from genomic DNA by LD-PCR.

利用长距离PCR从稻瘟病菌附着胞cDNA文库中克隆了mnh6和mtp1基因的cDNA序列，并从基因组DNA中克隆了mnh6和mtp1基因的全长DNA序列。

Due to decay in the Asgard genome, a result of excessive cloning, they no longer possess the ability to reproduce sexually, and all Asgard that were still alive at the time of their self annihilation were clones .

由于衰变在 asgard 基因组中，因过度的克隆，因此他们也就不再具备能力复制性，以及所有 asgard 认为仍有生还在的时候，他们的自我湮灭被克隆。

Abstract］ Objective To explore the effect of radiotherapy on the auxesis of Hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.Methods After radiotherapy with different doses,the Hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.

目的 探讨放射对宫颈癌Hela细胞系再增殖的影响，宫颈癌是否有必要实行非常规分割放疗提供定的理论基础方法在不同剂量的放射作用下，以台盼蓝染色细胞计法分析Hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

Abstract］ objective to explore the effect of radiotherapy on the auxesis of hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.methods after radiotherapy with different doses,the hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.results （1）tpot shortening was observed in hela cell after radiotherapy.

目的 探讨放射对宫颈癌hela细胞系再增殖的影响，为宫颈癌是否有必要实行非常规分割放疗提供一定的理论基础。方法在不同剂量的放射作用下，以台盼蓝染色细胞计数法分析hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。