克隆

We established the techniques of screening the plasmid expression library and cloning the immunogen genes.

建立了筛选质粒表达文库和克隆免疫原基因的技术方法。

Polyclonal anti-cTnI antibodies with high specificity and affinity were prepared by using purified recombinant cTnI as immunogen.

应用该方法成功制备了人cTnI的多克隆抗体，为进一步研究cTnI的结构与功能打下基础，并为有效制备高特异性的单克隆抗体提供借鉴。

The expressed fusion protein and GST-VP3 were purified, and used as an immunogen to raise GST-VP3-specific polyclonal antibodies in male rabbits.

纯化表达的GST-VP2e融合蛋白，利用其作为免疫抗原，制备了兔抗GST-VP2e融合蛋白多克隆抗体，其效价达到1：512，000。

This one is not a true clone, but a clone impostor.

这辆车还不是一个真正的克隆，只是一个冒牌。

The model of network with imprecise information and the corresponding solutions are researched in this dissertation.

基于非精确状态信息的网络模型，提出了非精确信息克隆组播路由算法。

An enriched PCR fragments with less complexity were obtained and mainly expressed in the denervated hippoampus After cloning into the plasmid vector, 46 clones were initially screened and subjected to autosequencing By comparison with the EMBO gene databases, 21 gene fragments of different types were obtained with 11 similar to genes of known function and 10 similar to ones of unknown function It is inferable that the altered gene expressions of hippocampus following the entorhinally denervation may underlie the specfic reinnervation and the formation of synapses by transplanted entorhinal neurons with host neuropil within the denervated target areas.

初步挑选出的46个转化后的阳性克隆，经点杂交筛选和基因序列分析，与Gene Database同源性比较后，得到21种基因片段，其中功能已知的有11种，功能未知的有10种。结果表明海马在去内嗅皮层后某些基因表达的改变，这可能是移植胚胎内嗅皮层神经细胞轴突特异支配去神经靶区和重建突触联系有关。

Because current federal law already forbids the use of federal funds to create embryos (the earliest stage of human offspring ago birth) for research or to 清楚ingly endanger an embryo's life, NBAC will remain silent on embryo research.

因该现今的联邦国家律法已经禁止应用联邦基金克隆胚胎(人类后裔在出生前的最早阶段)用于研究或者有意地威胁胚胎的生命，NBAC在胚胎研究上将保持沉默。

Because current federal law aleasysty tobids the use of federal funds to create embryos (the earliest stage of people offsummer ago birth) to research or to 清楚ingly endanger an embryo's life, NBAC be to remain silent on embryo research.

因该现今的联邦国家律法已经禁止实际运用联邦基金克隆胚胎(人类后裔在出生前的最早阶段)用于研究或者有意地威胁胚胎的生命，NBAC在胚胎研究上将保持沉默。

The "civilian" clones of Ingram models were made in a great variety of modifications, with minor differences in almost every detail.

的"平民"的模式克隆英格拉姆在种类繁多的提出在几乎每一个细节。

Our interprocedural analysis framework integrates interprocedural inlining, procedure cloning and complete analysis into an elegant one.

该框架还可以将过程间的嵌入式分析、克隆式分析和完全式分析统一起来。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。