克隆

This article reviews the research on the properties of organophosphorus acid hydrolase, and its gene isolation, cloning and genetic engineering.

综述了有机磷农药降解酶及其特性，以及相关基因的分离克隆和基因工程研究进展。

Objective: To clone and analyze the nucleoside triphosphate hydrolase gene of Toxoplasma gondii.

目的：克隆并分析我国刚地弓形虫三磷酸核苷水解酶的编码基因。

Objective:To clone and analyze the nucleoside triphosphate hydrolase gene of Toxoplasma gondii.

大 目的：克隆并分析我国刚地弓形虫三磷酸核苷水解酶的编码基因。

According to the sequence of the hydrolase gene, we designed the primers to clone the unknown sequence by rapid amplication of cDNA ends.

根据筛选所获得的其中一个阳性克隆水解酶基因的序列，设计引物，通过cDNA末端快速扩增的方法扩增基因的未知序列。

Objective To express human phospholipid hydroperoxide glutathione peroxidase in eukaryotic cells and to prepare its polyclonal antibody.

目的在真核细胞中表达人磷脂氢谷胱甘肽过氧化物酶并制备抗PHGPx多克隆抗体。

Keywords: Phopholipid hydroperoxide glutathione peroxidase; Cloning; Expression; Coxsackievirus of group B.

中文关键词：磷脂氢谷胱甘肽过氧化物酶；克隆；表达；克萨奇B组病毒；抗病毒作用

Its hypothalamic receptors are integral components of a complex physiological system involved in regulating fuel stores and energy expenditure.

Leptin是一种内分泌激素（167个氨基酸），主要在脂肪组织中表达，通过下丘脑对机体内能量的消耗和摄取起着重要的调节作用。ob基因的发现和克隆是体重调控机制研究的重大突破，揭示了脂肪组织具有内分泌的重要功能。

After six rounds of selection in medium containing mycophenolic acid, xanthine and hypoxanthine, we obtained purified recombinant viruses.

提取purified-rHVT感染细胞总DNA电转化大肠杆菌DH10B感受态细胞，在氯霉素抗性平板上筛选阳性克隆，并用酶切和PCR方法对其进行鉴定。

Objective:To study the hypoxanthine phosphoribosyl transferase gene mutation of rat peripheral blood lymphocytes and spleen lymphocytes induced by intermal radiation and of human peripheral blood lymphocytes induced by Y -ray.

目的：本实验主要是用克隆法研究放射性核素内照射诱发Wistar大鼠外周血淋巴细胞和脾淋巴细胞次黄嘌呤鸟嘌呤磷酸核糖转移酶基因突变及γ射线外照射诱发健康成年人外周血淋巴细胞HPRT基因突变情况。

The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody.

纯化的融合蛋白免疫新西兰大白兔制备多克隆抗体。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。