克隆

Objective To clone Horseradish peroxidase isozyme C3（HRPC3） in order to express the gene in vitro.

目的克隆辣根过氧化物酶同功酶C3（HRPC3）基因，为此基因的表达做准备。

Objective:To clone and express the horseradish peroxidase isozyme C3(HRPC3) gene.

目的：克隆与表达辣根过氧化物酶同功酶C3(HRPC3)基因。

This word was used in horticulture at first.

在这10 0多年的应用过程中，克隆的含义发生了显著的改变。

Galactosyltransferase 1 is the first cloned glycosyltransferase. It had been regarded as a house-keeping gene for a long time. In our previous investigation, it was found that GaT I was an intraction protein of SGT, and EIAF, one of the Ets family members could regulat its expression.

1，4半乳糖基转移酶是一个最早克隆的糖基转移酶，长期以来被认为是一个管家基因，本实验室以前的工作表明GalT Ⅰ是SGT的一个相互作用蛋白，Ets转录因子家族的成员EIAF可调控其基因的转录。

In this paper, three gene S-adenosylmethionine decarboxylase, ornithine aminotransferase and betaine aldehyde dehydrogenase plus one house-keeping gene had been cloned and their expression levels under different stresses were evaluated by real-time PCR technique.

已克隆S-腺苷蛋氨酸脱羧酶、鸟氨酸氨基转移酶、甜菜碱脱氢酶3个基因和内参基因的基础上，应用定量PCR技术，对这3个基因在不同胁迫时间下的表达水平进行分析。

I love my hu*and so much that I want six copies of him.

我爱他，我想拥有他的六份克隆。

Located at the mouth of the Hudson River, New York City is the busiest port of the country.

在过去的五年里，美国科学家一直在研究克隆技术。

Methods: The sequence of open reading frame of human ADH was cloned by RT-PCR from human liver RNA.

用RT-PCR技术从人肝RNA中克隆ADH开放阅读框架序列，并与表达载体p 。。。

Many problems regarding human reproduction or medicine may be solved with human cloning.

人的繁衍和医学方面的很多问题可以通过克隆来实现。

Balb/c mice were immunized with the purified PRRSV - BJ isolate. mouse spleen cell were fused with myelomas NSo .culture supernatants of hybridism's were screed by indirect ELISA. Positive colonies were cloned three times by limited dilution. One cell line named 6PR, which could secret McAb stably was obtained.

用纯化的猪繁殖与呼吸系统综合征病毒免疫Balb/c小鼠，应用淋巴细胞杂交瘤技术，取脾细胞与NS0骨髓瘤细胞融合，经间接ELISA筛选，三次有限稀释法克隆，得到一株能稳定分泌抗猪繁殖与呼吸系统综合征病毒单克隆抗体的杂交瘤细胞株：6PR。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。