克隆

Based on the sequence of the codominant RAPD marker〓,we designed SCAR primers SCN11 to amplify distinct band〓 with the same size as RAPD marker 〓.

根据克隆片段〓和〓的序列设计合成了一对SCAR引物SCN11，SCN11在95-5383中扩增出与〓大小相同的片段〓，在HB1中扩增出与〓大小相同的片段〓，在F1同时扩增出〓。

Protoscoleces of cyst were recovered from the brain of sheep naturally infected with coenurus of Taenia multiceps and total RNA was extracted.T.multiceps thioredoxin peroxidase cDNA fragment was amplified by RT-PCR from the total RNA,ligated into pMD18-T vector and sequenced.

从自然感染的病羊脑内采集多头蚴原头节，提取总RNA，采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶基因片段，PCR产物连接到pMD18-T载体，转化至大肠杆菌DH5α后测序，发现克隆到的TmTPx基因片段开放阅读框为453bp，编码150个氨基酸。

Cry3Aa7 wasthe first cloned cry gene highly toxic to coleopteran order in China.

这是目前国内分离克隆的第一个对鞘翅目害虫高毒力的Cry基因。

C1oning ofinsecticidal novel cry\' genes to Coleopteran pest tYom Bacillus lhuringiensisisolates will be beneficial fOr app1ication of pesticidal novel gene resources andwill lessen the gap betWeen domestic and abroad.

从Bt菌中分离克隆杀鞘翅目害虫的特异基因，将有效开发利用我国丰富的杀虫基因资源，缩小国内外在Bt杀虫基因基础研究领域中的差距，为工程菌和抗虫转基因植物的研制提供新的基因来源。

The highest level of expression was observed in the stem and leaf tissues and no obvious signal was detected in the root and coleoptile tissues.

通过RT-PCR和对Rht近等基因系的抑制缩减杂交，我们还克隆了14个有明显开放读码框的EST序列。

The gene has been expressed both in E. coli and in eukaryotes.

克隆的基因在大肠杆菌BL21中诱导表达。

Study on Cloning the Gene of β-1, 3-glucanase in Tea Plant and Its Expression in Escherichia Coli

论文标题：茶树β-1，3-葡聚糖酶基因的克隆及原核表达分析

I can still remember the smell of the cologne she used.

我还记得她用的克隆水的味道。

Molecular analysis by PCR and Southern blotting confirmed that the color patterns as expressed by cell colonies could represent the actual molecular events.

最后对部分克隆进行了PCR和DNA印迹鉴定，分子鉴定结果与发光的表型状况一致。

Target DNA fragments were amplified by polymerase chain reaction from the liver tissue of Columba livia using a pair of specific primers, and the amplified PCR products were cloned into pMD18-T vector.

利用特异引物，通过聚合酶链式反应(polymerase chain reaction， PCR)技术，从家鸽肝脏组织的总DNA中扩增到目的片段，并将扩增产物克隆到pMD18-T载体中，经菌落PCR与酶切鉴定、序列测定及序列分析。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。