克隆

Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究，建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序；此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作；采用植原体16SrRNA基因通用引物（R16mF2/R16mR1及R16F2/R16R2）和/或植原体核糖体蛋白基因引物（rpF1/R1）对所采集到的病害标样总DNA进行PCR扩增，根据植原体特异扩增条带的出现，共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害，初步明确了云南省植原体病害的种类；通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列（已在GenBank中共登录近20余个相关序列），通过序列比较分析，明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性；对一些发生在草本植物上的植原体进行了株系保存；在国内核心期刊及一般期刊发表研究论文 9 篇，申请技术发明专利1项；初步建立了有关植原体病害的网页；培养研究生4名。

It is understood that, at present, domestic and overseas markets Puzzle has thousands of toys, including more effective Puzzle toys only 200 variety, so that a large number of products, the cloning of foreign and traditional Chinese Puzzle toy products are more uneven product quality, lack of appropriate standards and local brands, has hampered China's development of Puzzle toys.

据了解，目前，国内和海外市场的益智玩具有数千，包括更有效的益智玩具，只有200多种，这样，大量产品，外商的克隆与传统益智玩具产品更为产品质量参差不齐，适当的标准和本地品牌缺乏，妨碍了中国的益智玩具的发展。

Since the success of cloning and expression of HCV genome fragment by researchers in Chiron corporation in 1989, many studies have been done on the epidemiology of HCV infection.

自1988-1989年chiron公司的研究人员克隆HCV的基因片断并获得表达抗原以来，世界上关于HCV研究的报道已有很多。

And then, the recombinant plasmid was introduced into the component cell E.coli JM109, which transformed by CaCl2 method, and the transformant was selected on M-H agar plate supplemented with 50μg/ml of chloromycetin and 60μg/ml of ampicillin.

将新酶全编码基因克隆入表达载体pHSG398，转化于感受态细胞大肠埃希菌JM109中，用加有抗菌药物的琼脂平板进行转化株筛选。

Objective: To study the expression of murine stem cell factor in CHO cells.

目的：克隆造血干细胞因子并在CHO 细胞中表达。

Post your question in the comments section for that lesson, and a CLO teacher will be happy to answer you.

邮政，你的问题在评论中科教训，并克隆老师会很乐意回答你们。

This is one man that i would clone, if i'm able to.

这是一个男人，我会克隆，如果我能够。

But it's still a clone.

但它仍然是一个克隆。

You cannot clone it, but you can buy it

你不可以克隆，但你可以买

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。