克隆

There are more than 20 key genes been cloned, which are possible to genetically manipulate carotenoid biosynthesis pathway in plants.

目前已成功克隆了类胡萝卜素生物合成途径中的20多种关键酶基因，为改良作物中的类胡萝卜素含量奠定了基础。

Total RNA was extracted from flowers of cattleya, according to the conserved acid sequence for ACC oxidase in other orchids, we designed a pair of oligo nucleotide primers. Using RT-PCR method, a cDNA fragment about 967 base pair, which encoded 321 predicted amino acid residues, was amplified.

以卡特兰花瓣为试材，提取其总RNA，并根据其他兰花的ACC氧化酶(1-aminocyclopropane-1-carboxylic acid oxidase， ACO)基因保守序列设计一对特异性引物，通过RT-PCR法克隆得到1条967bp的卡特兰ACO cDNA片断，共编码321个氨基酸残基。

An antisense expression vector of the cattleya-ACO gene, which named pBI121ACC, was constructed, and the autisense sequence was controlled by the CaMV 35S promoter. The work laid the foundations of the future transgenic research for prolonging the flowering period of the transgenic Cattleya via autisense technology.

将克隆的卡特兰ACO片段反向连接到植物表达载体pBI121中CaMV35S启动子的下游，构建了卡特兰ACO基因的反义表达载体pBI121ACC，为进一步应用反义技术培养长花期卡特兰新品种奠定了基础，也为首次应用生物技术延长卡特兰花期做出了尝试。

Growth of cells were observed daily by MTT assay, the status of cell proliferation were observed by Plate colony formation, and the cell cycle of the cells were observed with flow cytometry .

应用MTT比色法绘制细胞生长曲线；克隆形成实验了解细胞增殖情况；流式细胞仪观察细胞周期。

The gene of ESAT-6 from genome of bovine mycobacterium tuberculosis strain that from cervine tuberculosis was amplified by polymerase chain reaction and inserted into pGEM-TE vector. After sequencing, we analyzed the gene encoding ESAT-6 protein with molecular biologic software.

从患结核病鹿的肺组织中粗分离牛分支杆菌制备DNA模板，用PCR对牛分支杆菌ESAT-6基因进行了扩增，将扩增的产物连接于测序载体pGEM-TE上克隆，经测序确定无误；并使用分子生物学软件对ESAT-6基因进行了分析。

The number of colony-forming cells increased from 16 + 2 to 26 + 3 CFCs/100,000 monocytes plated.

每100，000单核细胞中克隆形成细胞数从16±2上升到26±3。

Using Cassia tora as material,a cDNA encoding chalone synthase was cloned from tender leaves of Cassia tora by RT-PCR and RACE.The full-length cDNA of CHS from Cassia tora had 1 459 bp with an open reading frame encoding 390 amino acids of protein.

以决明为实验材料，利用RT-PCR和RACE技术，从决明嫩叶中克隆出查尔酮合成酶(Chal-one synthase，CHS)基因，其cDNA全长为1 459 bp，编码一个由390个氨基酸残基组成的多肽。

The first part of the thesis is the molecular cloning and characterization of LAG3Hs gene.

本论文第一部分是LAG3Hs基因的克隆和初步的功能研究。

This parameter indicates if the cloned node should include all attributes and child nodes of the original node.

这个参数指明了被克隆的节点是否应该包含所有的属性以及原始节点中的子节点。

Chinaberry tree samples showing witches' broom were collected in Danzhou, Hainan Province. Using universal primers Rl6mF2/Rl6Mr1 for phytoplasma 16 S rDNA, a fragment of about 1.4 kb was amplified, cloned and sequenced from the DNA samples extracted from the diseased Chinaberry tree.

利用植原体16S rDNA通用引物对Rl6mF2/Rl6mR1，通过PCR技术从表现典型丛枝症状的苦楝植株总DNA扩增到约1.4 kb的特异片段，将此片段克隆后进行序列测定、分析及系统关系树构建。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。