克隆

The results indicated that CIRP in the organisms could be induced at low temperature and might protect the organisms from the cold damage. The amino acid sequences deduced via cDNA clone were 100%, 99.4%, 95.5%, 67.4%, 58.4%,76.9%, and 79.1% identical to those of the CIRP in mice, rats, human, bullfrog and axolotl cells, and Xenopus embryos and oocytes, respectively.

根据克隆的cDNA所推测的氨基酸序列与GenBank上公布的小鼠、大鼠、人类、牛蛙、美西螈、非洲爪蟾胚胎细胞和卵母细胞的CIRP氨基酸序列同源性分别为100%、99.4%、95.5%、67.4%、58.4%、76.9%和79.1%。

No Ayu or Nayuki clones and yet it still has its own unique style.

没有阿尤和名雪的克隆版，但是它依然保有它自己独特的风格。

Objective To construct a cDNA library for Babesia orientalis and screen immunologically positive clones. Methods Total RNA of B.

摘要目的构建东方巴贝虫cDNA文库，从中筛选免疫学阳性的克隆。

One cDNA clone was identified by immunoscreening from a cDNA expression library prepared from Babesia gibsoni merozoite mRNA and its complete nucleotide sequence was 2108bp in length,including one single open reading frame and encoding a 62ku protein which had high homology with that of apical membrane antigen-1(AMA-1) of other species.

通过免疫筛选法，从构建的犬吉布森巴贝虫cDNA基因表达文库中筛选到1个cDNA克隆，此cDNA核苷酸全长2108bp，包含1个完整的开放阅读框，编码蛋白的分子质量约为62ku。

The objective of this research is to clone the expression element of a major secretory protein gene from Bacillus sp.

本研究的主要目的是克隆芽孢杆菌胞外高丰度蛋白的表达元件，为今后构建表达载体奠定基础。

Objective To clone and identify a protease gene from a novel Bacillus subtilis.

目的 从新分离的枯草芽孢杆菌中克隆和鉴定蛋白酶基因。

Its alterations of phenotype and structure have been further studied at whole, organic and cellular levels. By means of backcross between wildtype and the mutant, its genetic background has been analyzed.

本文在整体水平、组织水平和细胞水平上深入研究了该突变体的表型和结构的变化、通过突变体与野生型之间的杂交对其遗传背景进行了分析，并运用分子生物学手段对突变体相关基因进行了克隆和定位，初步探讨了该基因的表达模式和功能。

Monoclonal and Polyclonal Antibodies Courtesy of Alex Bagley. Used with permission.

单克隆抗体和多克隆抗体由Alex Bagley提供，使用得到其允许。

Some think that cloned human beings will be deprived of freedom or dignity or personal identity.

一些人认为被克隆的人将被剥夺自由，尊严或个人的身份。

T cells can be isolated from fresh human plaques, cloned, expanded in culture, and challenged with candidate antigens.

从新鲜的人体斑块中分离的T细胞，在体外克隆、扩增后，与各种候选抗原进行反应。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。