克隆

The constructed pcDNA3-IL-15 plasmid was identified by Bgl Ⅱ digestion,PCR amplification and sequenceanalysis respectively,to ensure that the hIL-15 cDNA was properly inserted into thevector and the sequence was correct.Then the pcDNA3-IL-15 plasmid was instantlyexpressed in COS-7 cells by means of lipofectamine transfection,and followed by astable expression in Chinese hamster oval cells.8 positive cell colonies wereobtained after the G418 selection,designated CHOA~H cells.

经过BglⅡ酶解分析，PCR扩增鉴定和DNA测序分析，证明IL-15 cDNA已正确插入克隆载体且序列正确后，采用脂质体法，首先将pcDNA3-IL-15重组质粒转染至非洲绿猴肾细胞（COS-7）做瞬时表达，再转染至中国仓鼠卵巢细胞进行稳定表达。

Coli . The clones were verified by restriction enzyme digestion and PCR. Sequence and homology analyses showed that this phytoplasma strain shares high homology (99.0%) with the phytoplasma of Aster Yellows that belongs to 16SrI group.

通过酶切、PCR鉴定，对筛选得到的重组阳性克隆进行核酸序列测定及同源性比较分析，结果表明其与植原体16SrⅠ组中的西方翠菊黄化植原体同源率为99%。

In the spring of 1996, a book was published telling the supposedly true story of an elderly millionaire who, at great expense, succeeded in producing a clone--an exact genetic copy of himself.

1996年的春天，已经发行的一本书讲述一个据说是真实的故事，一个老年的百万富翁花费巨资，成功的生产出一个克隆，一个完全的他自己的基因复制。

This is a clone of the Original Frogger by Atari.

这是原始由Atari Frogger的克隆。

A novel gene CCR (Cell-Cycle Related) was cloned from Atriplex hortensis. Northern blot analysis showed that the CCR gene specifically expressed during G1 and early S phase of the cell cycle, but suppressed by ABA treatment.

从山菠菜和拟南芥中克隆了一个新的未知功能的植物基因，编码DNA结合蛋白，其在细胞周期的G1和S期特异表达，受ABA抑制，命名为CCR（Cell-CycleRelated）。

Genes encoding Drought-Responsive-Element Binding proteins were cloned from Triticum aestivum and Atriplex hortensis. TaDREB1 and AhDREB1 can specifically bind the DRE elements in the promoters and thus activate the transcription of downstream genes in yeast cells as well as transgenic Arabidopsis and transgenic tobacco plants.

克隆了小麦和山菠菜转录因子TaDREB1和AhDREB1基因，它们都编码DRE（Dehydration-Responsive-Element）结合蛋白，在酵母单杂交系统、转基因拟南芥和转基因烟草中都能特异结合DRE元件并激活下游报告基因的表达。

Combine the advantage of immune clone algorithm and imitate anneal algorithm, put forward task of a kind of reseau to attemper optimize model and algorithm.

结合免疫克隆算法和模拟退火算法的优点，提出了一种网格任务调度优化模型和算法。

Objective: Augmenter of liver regeneration cloned from liver of weaning rat is a novel hepatic stimulator. It was reported that ALR mRNA expression was correlated with DNA synthesis of hepatocytes after partial hepatectomy, and could significantly promote the survial rate of intoxicated hepatitis rats.

目的：肝再生增强因子是从新生大鼠肝组织中克隆到的一种肝增殖刺激因子，其特异mRNA的表达与大鼠肝部分切除后肝细胞DNA合成密切相关，是一种特殊的促肝细胞分裂原，能特异地刺激肝细胞增殖，是肝再生的重要调控因子，能提高中毒性肝损伤动物的存活率，在肝损伤修复过程中发挥作用。

Four positive clones were obtained by the selection of autotrophic phenotype and β-galactosidase assay.

利用营养缺陷型和β—半乳糖苷酶活性筛选到4个阳性克隆，测序结果表明它们具有相同的序列。

We pass to make with network coping etc. technique in practice its proceed more quickly and availably.

我们在实习中通过用网络克隆等技术使其更为快速有效地进行。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。