克隆

According to recent researches, intracellular signalling pathway systems involved in appressorium differentiation is related with cAMP pathway and MAP pathway. In this research, 21 bacteria strains and Chaetomium cupreum strains were assayed for their antagonism to appressorium formation in M.grisea.

随着稻瘟病菌一系列致病基因的克隆及功能明确，附着胞形成的信号传导途径包括：cAMP途径和MAP途径，这些途径中基因的失活都影响附着胞的形成。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

After positive clones were induced by L arabinose for 4 hours, the purity of protein was detected by SDS PAGE and its bioactivity was identified by competitive inhibition ELISA test.

阳性克隆用左旋阿拉伯糖诱导4 h， SDS PAGE电泳检测蛋白纯度，竞争抑制ELISA实验检测其活性。

Induced with arabinose, the pBAD/hIL-10 produced hIL-10 in periplasmid space of E.

培养筛选出的阳性克隆，应用阿拉伯糖诱导表达hIL-10，并进行初步纯化。

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

Objective: To study cloning expression of human arsenite resistance related gene.

目的 ：克隆并表达人类抗砷基因 human arsenite resistance related gene。

Does this article from the square design, the city layout, how in the shore water landscape design discuss in the concrete design process, has the local characteristics factor to integrate to the design, the constitution has uniquely, and is any place is unable to duplicate and the clone this kind of cultural artware.

本文从广场设计、城市设计、滨水景观设计中探讨如何在具体的设计过程中，把具有地方特色的因素融入到设计中来，构成具有独一无二的，并且是任何一个地方都无法复制的和克隆的这样一件文化艺术品。

Cloning and Functional Analysis of mnh6 and mtp1 Genes in Rice Blast Fungus, Magnaporthe grisea Abstract Rice blast is a severe disease to harm rice and the rice blast fungus is a well-known ascomycete Magnaporthe grisea.

稻瘟病菌mnh6和mtp1基因的克隆和功能分析摘要稻瘟病是一种严重危害水稻的毁灭性病害，其病原菌为子囊菌Magnaporthe grisea。

The successful cloning technology, the birth of lives into the asexual reproduction of the times.

克隆技术的成功，使生命的诞生进入无性繁殖的时代。

As a result of having cloned themselves for so long, the evolutionary process necessary to ascend is no longer possible for the Asgard.

由于具有克隆自己这么久，在进化过程中，要继承皇位的是不再可能为

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。