克隆

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Clonal anergy and suppressor cells are main mechanisms of the tolerance.

克隆不应答和抑制细胞是耐受的主要机制。

Inhibition of this pathway results in development of antigen-specific unresponsiveness and clonal anergy.

阻断该通路可以导致抗原特异性的免疫耐受和克隆不应答状态。

We propose that induction of T cell anergy may depend on the T cell growth, activation and differentiation state or cloning conditions.

结果提示，体内T细胞与克隆T细胞存在功能上的不同，体内T细胞的无能诱导可能需要不同的条件。

Most of these cells are clonally deleted in the bone marrow, but some of these cells avoid deleting and escape to periphery by anergy and produce autoantibody.

但有些B细胞通过免疫无力的方式逃脱了这种克隆删除到达外周，产生抗自身的抗体。

Projects included: Cloning and functional studies of diabetes related genes;Structural studies of thermostable Catechol 2,3-Dioxygenase by anomalous scattering; Prevention and cure, and influence factor studies of major child bearing blemish of national minority in Guizhou province.

该课题组的主要研究方向包括大规模高通量人类基因克隆及功能研究；基因芯片技术、生物信息学及功能基因组学研究；蛋白质晶体结构与功能；蛋白质热稳定机制；疾病相关基因的研究。

Objective To clone and analyze the innate immunity-associated genes differentially expressed in Anopheles dirus.

目的 克隆及分析与先天免疫相关的大劣按蚊差异基因。

In this study, a feedback insensitive anthranilate synthase gene ( ASA2 ) cloned from a tobacco cell line was tested for Agrobacterium mediated transformation of axis tissue of soybean mature embryo, with a tryptophan analogue 5 methyltryptophan (5 MT) as the selective agent.

通过从烟草中克隆的邻氨基苯甲酸合成酶基因(ASA2 )作为筛选标记基因，并采用氨基酸的类似物 5 甲基色氨酸为筛选剂，进行了农杆菌介导的大豆成熟胚尖转化研究。

TRP1 gene of Candida glycerinogenes (CgTRP1), encoding phosphoribosyl anthranilate isomerase(PRA1) was cloned by complementation of the trp1 mutation of Saccharomyces cerevisiae W303-1A.

本文采用遗传互补的方法从产甘油假丝酵母基因文库中克隆了TRP1基因(CgTRP1)。

Cloning, expression and antigenic analysis of heat shock protein A gene of human Helicobacter pylori.

人幽门螺杆菌热休克蛋白A编码基因的克隆、表达及抗原性研究。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。