克隆

A novel rapid alkalinization factor gene of litchi was isolated from cDNA library of litchi pericarpGenBank accession No.

从已构建的荔枝果皮cDNA文库中挑取克隆测序，获得一条荔枝快速碱化因子基因（GenBank登录号：EU024484）。

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

This is the default clone allocator used by all pointer containers.

这是被所有指针容器使用的缺省克隆分配器。

Furthermore, the clone allocator might be allowed to have state.

此外，克隆分配器可能允许具有状态。

For that reason, this library makes use of the concepts in Boost and exposes the following handy Clone Allocator classes.

出于这个原因，使得这个图书馆在提高概念的使用和公开以下方便的克隆分配器类。

We established the technical system to obtain the EST library of plant specific chromosome,which is important to gain the ESTs and genes located on specific chromosome and provide a new tools for complicated allopolyploid genome research.

本项目获得了建立植物特定染色体EST文库的技术体系，它对分离特定染色体的EST及克隆新基因有重要参考价值，也为异源多倍体植物基因组研究提供了新的技术手段。

First, nucleic acid sequence encoding ectodomain of AMAl was amplified from Pf genome and, was cloned into prokaryotic expression vector.

首先，自P.f基因组DNA中扩增出编码AMAl胞外域的基因片段，经测序确证后，克隆入原核表达载体。

The development of Amanita manginiana in the study site, from sexual spores to mature sporocarps, might take more than one year.

在对隐花青鹅膏菌连续两年的种群结构进行了研究中，种群的每一个子实体也均来自不同的克隆。

The light and heavy chain variable region gene of the monoclonal antibody had been cloned and sequenced. VH region gene consisted of 350 bp, and VL region gene consisted of 321 bp encoding 107 amine acid residues .

采用分子生物学方法，对抗RB单克隆抗体的轻重链可变区基因进行克隆及序列分析，重链约350bp，轻链为321bp，编码107氨基酸。

We propose experimentally feasible schemes to implement the optimal asymmetric economical 1→2 and 1→3 phase-covariant cloning,which work without ancilla,in the context of ion-trap system.

我们提出利用离子阱技术来实现1→2和1→3的最优非对称经济型相位协变克隆的实验可行性方案。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。