克隆

Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物，以新生5d的西农萨能羊皱胃组织总RNA为模版，通过RT-PCR方法获得凝乳酶原前体cDNA，克隆测序后进行序列比对。

Keywords : pomp18 gene;clone;expression;Chlamydophila abortus

中文关键词： pomp18基因；克隆；表达；流产嗜性衣原体

Objective To clone the BP26 gene of B. abortus, highly express in K. coli and purify the expressed product. Methods Extract the genomic DNA of B.

目的 克隆布鲁氏菌BP26基因，并在大肠杆菌中高效表达及纯化BP26蛋白。

Key words: Brucella abortus;omp25 gene;cloning;prokaryotic expression

流产布鲁氏菌；omp25基因；克隆；原核表达

Swine;Chlamydophila abortus; omp1 gene;clone; prokaryotic expression

猪；流产嗜性衣原体；omp1基因；克隆；原核表达

In order to cluone, seauence and express the outer membrane protein 25 (Omp25) gene sequence of wapiti's Brucella abortus strain ML.

目的 克隆马鹿布氏杆菌ML分离株25kDa外膜蛋白(Omp25)基因，并在大肠杆菌中表达。

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

An Automatic Refactoring Method of Cloned Code Using Abstract Syntax Tree and Static Analysis.

使用抽象语法树和静态分析的克隆代码自动重构方法。

The acidophile s composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis.

采集了酸性矿山废水区域酸性沉积物样品，运用16S rDNA克隆文库技术和变性梯度凝胶电泳技术，研究了沉积物中的嗜酸菌类群以及它们的群落结构。

Researches on synthesis pathway of plant oil and characteristics of acyl carrier protein gene are summarized systematically.

植物油脂作为主要的食用油，其生物合成途径及相关基因的克隆已成为研究热点。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。