克隆

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

Chimeric clone strategy has proved to be successful in rescuing CRF07_BC proviral genome,which was a straightforward,feasible and highly efficent method.

LTR功能和全长克隆策略对感染性克隆构建至关重要，pNL4-3的LTR能成功用于拯救CRF07_BC毒株前病毒基因组。

Expression vectors pET-TPO and pBV-TPO were constructed to express full-length TPO cDNA in E.coli . Influence of SD-AUG and promotors on the expression level was studied.

在克隆并分析了TPO cDNA的基础上，将全长hTPO cDNA克隆到原核表达载体pET-15b和pBV220中得到了表达，并且以pBV220为载体探讨了SD序列与AUG之间的距离对cDNA表达效率的影响；为了进一步提高表达效率，将hTPO cDNA 5？

Honghezi,Dongbi,Shieryuelongyan,etc.The results showed that the method of Calcium-alginate -embedded suspended culture shaking at50r/min was suitable for longan proto plast culture;and the optimal regeneration,with up to5%of microcolony formation,occurred when protoplasts from suspensions were cultured ...

研究结果表明，海藻酸钙包埋悬浮振荡培养(50r/min)是龙眼悬浮细胞原生质体培养的适宜培养方式，在含有多种生长调节剂的改良MS培养基(MP6)上，再生小克隆的形成频率可高达5%；采用龙眼胚性愈伤组织体胚诱导、成熟和萌发的优化方案，原生质体再生小克隆分化子叶形胚状体的频率达100%，萌发植株的频率一般大于45%。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

South Korea "cloning" father of cloning stem cells in the field and hwang's achievement is refers to concoct, the Seoul national university announced to investigate immediately.

韩国&克隆之父&黄禹锡在干细胞和克隆领域的成果被指造假后，其所在的韩国首尔大学即刻宣布对此事展开调查。

Beijing Fatty Chicken, Langshan Chicken and Small-tailed Han Sheep were selected as experimental material. Fibroblast library was constructed through tissue confluency culture and cell freeze technology. Growth dynamics, genetic stability and contamination of of cross breeds etc.were detected through growth curve, chromosome analysis, isoenzyme analysis etc. Cloning and sequence of gene GPAT of Beijing Fatty Chicken was analyzed by cloning technology and software related.

本论文以北京油鸡、狼山鸡、小尾寒羊为研究对象，采用组织块贴壁培养法和细胞冷冻技术构建成纤维细胞库，采用生长曲线、染色体分析、同工酶分析等方法对细胞生长动态、遗传稳定性、种间交叉污染等进行检测；采用基因克隆技术和相关分析软件对北京油鸡GPAT基因进行克隆和序列分析。

Within the most frequently mutated region on chromosomel7pl3.3 in human cancers, a novel human plasma membrane-associated gene, named CT120, was isol...

CT120是在染色体17p13.3高频突变区通过电子克隆及RACE方法克隆并鉴定出的一个人新基因。

Methods(1) Construction of plant expression vector containing RS geneThe cloning vector pT-RS was constructed containing RS gene extracted from the leaf tip of Vitis vinifera by PCR amplification and RS sequence was identified by PCR amplification,enzymes digestion and sequence analysis.The plant expression vector containing RS gene was obtained and RS sequence was identified by PCR amplification and double enzymes digestion too.

研究方法(1)含白藜芦醇合酶基因的植物表达载体的构建提取葡萄基因组总DNA，通过PCR扩增得到RS基因，将此基因连接到克隆载体pGEM-T Vector，得到重组载体pT-RS；经PCR、酶切及序列分析鉴定后，将RS基因克隆到植物表达载体pBI121，得到重组载体pBI-RS，用PCR及双酶切方法进行鉴定。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。