克隆

Further isomerization and substitution of this central intermediate leads to the synthesis of other flavonoids. CHSs has been isolated from different plant species, including both monocots and dicots, some of their genomic DNAs and/or cDNAs have also been isolated.

目前，包括单子叶和双子叶植物的很多物种中，有的物种查尔酮合成酶已经得到纯化且基因组、cDNA克隆得到分离，有的或者得到了纯化酶，或者分离得到了基因组、cDNA克隆。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

The phytase gene was cloned in this study would not only provided excellent gene resource which could express in the microbe and plant but also remedied the vacancy of phytase gene cloned in the funguses.

本研究所克隆的植酸酶基因不仅为植酸酶进一步在各种微生物、植物中表达提供了优良的基因源，而且弥补了食用菌中克隆植酸酶基因的空白。

Noah is the name given in advance to the clone of a dead gaur,a rare type of endangered wild ox found in India,Bangladesh and Southeast Asia.The new Noah is expected to be born any day now to Bessie,a cow living on a farm in Iowa.Cows have given birth to gaurs before,but this is the first time that one animal species is acting as surrogate mother to a clone of a different species.Advanced Cell Technologies,a small biotechnology company in Massachusetts,is using a novel cross-species nuclear-transfer technique that could usher in a new era in conservation.

诺亚是名字事先给予到克隆的死野牛，一种罕见的濒危野生牛发现，在印度，孟加拉和东南亚asia.the新诺亚，预计将出生的任何一天，现在刘湘文，牛生活在一个农场iowa.cows已经诞生gaurs之前，但是这是第一次有一个动物物种，是作为代母的一个克隆的一个不同species.advanced电池技术，一个小型生物技术公司，在美国马萨诸塞州，是用一种新型的跨物种移植核技术可以开创一个新时代，在自然保育。

The age class structure of Salix oritrepha population in alpine, cloudy and wet area of east Qilian Mountian is studied ,on two levels of genets and their ramets and sprouting branches .The results show that genets population is very short of the first and second class seedlings,and most of seedlings belong to the third and forth classes, and the development trend of population on this level is stable;on the other hand,the age structure of remets and sprouting branches population is more complete,and its age class distribution chart likes triangle, belonging to increasing type.In general,Salix oritrepha population in this area is increasing, but its reproduct way have changed in community succession process in order to accommodate varying circumstances.

从无性基株个体种群和无性克隆分株及萌生枝种群两个层次上对东祁连山高寒阴湿地区山生柳种群年龄结构进行分析，结果表明：无性基株个体种群 0~10 龄级幼苗贮备严重不足，11~20 龄级苗木所占比重最大，种群动态呈稳定型；无性克隆分株和萌生枝种群年龄结构比较完整，结构分布图呈正三角形，种群动态表现为增长型；总体上来讲山生柳种群呈增长趋势，只是在演替过程中为适应环境其繁殖方式有所改变，导致现阶段山生柳种群两个层次结构状态。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

Methods: Based on B-cell epitope 82~93, a multiple antigenic peptide system was synthesized as immunogen and C-57BL/6 mice were divided into 3 groups randomly. MAP group (n=5) referred to that C-57BL/6 mice were immunised with the immunogen and the polyclonal anti-serum was produced. The polyclonal antibody was identified by using an ELISA assay.

将C-57BL/6小鼠随机分为对照组、MAP组、人参总皂苷组，MAP组在预测MAGE-12的B细胞表位序列为QSDEGSSNEEQE(82-93)的基础上，采用4分枝多重抗原肽结构设计合成抗原肽，免疫C-57BL/6小鼠，产生多克隆抗体，应用ELISA实验检测多克隆抗体是否为人的抗原肽的特异性抗体。

Some of the great hopes for cloning include the ability to perform tra lants, whenever they are needed, using cloned orga .

人们对克隆的一些伟大希望包括在需要进行移植手术时，人们能使用克隆器官。

The transposon tagging and map based cloning is the major methods for disease resistance genes cloning in the past.

目前植物抗病基因的克隆方法主要有转座子标签技术和图位克隆技术。

In this study, we have cloned and identified two full-length cDNAs of OsBIMK2 and OsBISERK1, which encoding MAPK and SERK, respectively, and associated with benzothiadiazole induced resistance.

本文克隆和鉴定了水稻中与苯并噻二唑(benzothiadiazole，BTH)诱导抗病性相关的编码MAPK的OsBIMK2基因和编码SERK的OsBISERK1基因全长cDNA；研究了OsBIMK2基因和OsBISERK1基因在水稻—稻瘟病菌亲和与非亲和互作过程中的表达模式；原核表达了OsBIMK2基因，获得重组OsBIMK2蛋白，并研究了重组OsBIMK2蛋白的生化特性；克隆了OsBIMK2基因的启动子序列，并运用农杆菌介导的瞬间表达系统分析鉴定了启动子中参与基因表达调控的可能顺式元件；将水稻OsBIMK2基因导入烟草，获得过量表达OsBIMK2基因的转基因植株，这些转基因植株中防卫反应基因PR-1组成型表达，并提高了抗病性。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。