克隆

Only one complete corollinae chromosome was detected in Ml4, and was determined corollinae chromosome 9. We have used in situ hybridization of genomic DNA to discrimination the parental chromosomes in Beta Ml 4, suppression of cross-hybridization by blocking DNA was not necessary indicating that the investigated Beta genome contain sufficient species-species DNA enabling the unequivocal determination of genomic composition of the hybrids.In our study , the results of BAC -FISH combined with the analysis of GISH in Beta M14,indicating that present of the corollinae chromosome 9 alone was sufficient to confer apospory in Beta M14 upon celluar and molecular..

BAC克隆16-M11和26-L15携带M14花期特异表达的基因；在M14甜菜对应的有性栽培甜菜染色体上没有出现信号，呈半合子状态；包含的单或低拷贝DNA序列在野生白花甜菜进化过程是共线的；两个BAC克隆部分同源；位于末端重组抑制区，所有这些不同寻常的特性都暗示着：BAC克隆16-M11和26-L15和无融合生殖特性相关，可作为无融合生殖

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

从7种重要鲽形目经济鱼类——高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽和条鳎、夏鲆的脑垂体中提取mRNA，并采用RT-PCR方法，根据已克隆得到的鲆鲽鱼类生长激素基因的保守性序列设计特异性引物，克隆了含开放阅读框的生长激素(Growth Hormone，GH)cDNA序列，测序结果显示，高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽、条鳎和夏鲆的GH cDNA长度依次为479 bp、564 bp、519 bp、440 bp、564 bp、440 bp和522 bp，编码140-170个氨基酸的成熟GH多肽片段；从漠斑牙鲆和大菱鲆脑垂体中提取mRNA，利用SMART-RACE技术建立脑垂体cDNA文库，并从该文库中克隆出大菱鲆和漠斑牙鲆生长激素基因cDNA全长序列。

The coppiced shrub remained two coppice shoots adding the parameters obviously of the number, the total length,1 the biomass of new branch and the number and biomass of leaf. 3. the leaf area decreasing with the increasing of adjusting degree.

在相同的生境下，三种调控措施中对基径、顶梢生长影响最明显的是2克隆株/丛与3克隆株/丛；2克隆株/丛的调控措施明显增加当年新生枝的数量、总长度、生物量以及叶片的数量和生物量；而叶面积随调控措施程度的增加有逐渐减小的趋势。

There were no significant correlations between food concentration and the rate of population increase of Clone B as well as the mixis rate and fertilization rate of Clone A, B and C. However, the relationships between food concentration and the rate of population increase of Clone A and C were significantly curvilinear, with the regression equation Y=0.019X2-0.209X+0.638 and Y=0.042X2-0.402X+0.852, respectively. The relationships between the food concentration and the instant rate of population increase, the mixis rate and the fertilization rate of clone D were all significantly curvilinear, with the following regression equations Y=-0.052X2+0.609X-0.667,Y=0.994X2-15.763X+73.424 and Y=-0.251X2+15.940X-15.446, respectively.

克隆B的种群瞬时增长率、种群中的混交雌体百分率和混交雌体受精率均与食物密度间无显著的相关性；克隆A和C的种群瞬时增长率与食物密度间呈曲线相关，而其种群中的混交雌体百分率和混交雌体受精率均与食物密度间无显著的相关性；克隆D的种群瞬时增长率、种群中的混交雌体百分率和混交雌体受精率均与食物密度间呈曲线相关。

Based on the general principle of value, and by analyzing the instrument value and destination value of its study and application, this article is trying to disclose the diversity, the complication and the doubleness of its value, to point out the practical meaning of the study and to establish an evaluation system to judge it objectively and reasonably, thus imposing the necessary limit on its value. In this way, we can make the most of its positive side and avoid or dispel its negative side.

本文立足于价值的一般原理，通过对克隆技术研究和应用的工具价值和目的价值的全面剖析，揭示其价值的多样性、复杂性和正负两面性，指出对其价值限度研究的实践意义，并且尝试建构一套价值评判体系对克隆技术进行客观、合理的价值判断，以此实现对克隆技术价值的必要限度，使之最大程度地发挥正价值，避免或消解其负价值。

In this paper, degenerate PCR was used to amplify the DM domains of Dmrt genes in Gallus gallua.Domesticus.

通过简并PCR克隆技术，扩增和克隆了家鸡基因组中的DM结构域，经序列分析，获得了两个具有不同DM序列的克隆，分别命名为GgDmrt2和GgDmrt5。

IPTG induces the expression protein of GST-pUL49j and the induced product was purified electroeluting.The PAGE mortar contained GST-pUL49j was used as antigen, injected intradermally to immunize male New Zealand white rabbit to get polyclonal antiserum.

以含蛋白GST-pUL49j的PAGE胶泥做为抗原，皮内多点免疫注射雄性新西兰兔制备多克隆抗血清，ELISA测定多克隆抗血清效价，Western blot检测多克隆抗血清的特异性。

The similarity between 71.8% of 41 detected sequences and published clone sequences were less than 97%, which represented a new community and 15.3% of the clone sequences which identitied with GenBank clone sequences were under 85%, which may represent some new families or suborders.

在这些克隆序列中有71.8%的克隆序列同已知序列的相似性低于97%，属于放线菌的新类群，这些可能的新类群中有15.3%的克隆序列与已知菌株的相似性小于85%，这些克隆序列的分类地位都在科一级的分类单元上，有的可能分类地位更高。

Results Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class.

所获得的51个克隆序列属于39个OTUs，其中52.9%的克隆序列分布于放线菌门放线菌亚的5个亚目和酸微菌亚纲中，并且在这两个亚纲中有大量克隆序列属于放线菌的新类群，另外47.1%的克隆序列以极高的自展值在放线菌门内支持形成一个独立的大分支，极有可能代表一个新亚目或更高级分类单元的类群。

The resulting patchwork of laws, people on all sides of the issue say,plicates a nationwide picture already clouded by scientific and ethnical questions over whether and how to restrict cloning or to ban it altogether.

在克隆问题上各方人士认为，关于是否限制克隆、如何限制克隆或索性禁止克隆的科学及伦理问题，已经使全国性立法变得扑朔迷离。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。