克隆

Use pCR2.1-TOPO and pCR2.1-Blunt II TOPO vectors as models, we investigated the possibility to use TOPO T/A vector (pCR2.1-TOPO) to clone blunt-ended PCR product and to use TOPO blunt vector (PCR-Blunt II TOPO) to clone 3'-A PCR product.

以pCR2.1-TOPO与pCR2.1-Blunt II TOPO载体为模型，我们研究了利用TOPO T/A载体（pCR2.1-TOPO）克隆平端PCR产物与利用TOPO 平端载体（pCR2.1-Blunt II）克隆3'-A末端PCR产物的可能性。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二酰基甘油酰基转移酶(Diacylglycerol acyltransferase， DGAT)基因，硬脂酰-酰基载体蛋白脱饱和酶(Acyl- desaturase， SAD)基因克隆测序，经测序，通过NCBI与已知的核苷酸序列进行同源性比对，结果表明：克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二基甘油基转移(Diacylglycerol acyltransferase， DGAT)基因，硬脂-基载体蛋白脱饱和(Acyl- desaturase， SAD)基因克隆测序，经测序，通过NCBI与已知的核酸序列进行同源性比对，结果表明：克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

In the method, the polyclonal antibodies are obtained by utilizing syntactic quinoxalone antibiotics multi-cluster antigen immunization; the coupling compound of the half antigen of the quinoxalone antibiotics and an egg-white protein OVA is taken as an envelope antigen; the quinoxalone antibiotics is taken as a standard; the indirect competitive enzyme-linked immune detection method for the quinoxalone antibiotics in animal food is established; a rapid and high efficient detection means is provided for the residues of the quinoxalone antibiotics in the animal food; because the polyclonal antibodies are adopted, the cost is low, and the stability and the repeatability are good; the detection limit (IC is smaller than 90) is 0.0126 ng/ml, the median inhibitory dose (IC is smaller than 50) is 0.52 ng/ml, and the detection range (IC is smaller than 20 to 80) is 0.04467 to 14 ng/ml.

本发明利用合成的喹诺酮类抗生素多簇抗原免疫得到多克隆抗体，以喹诺酮类抗生素半抗原与卵清蛋白OVA的偶联物作为包被抗原，以喹诺酮类抗生素为标准品，建立动物性食品中的喹诺酮类抗生素的间接竞争酶联免疫检测方法；为喹诺酮类抗生素在动物性食品中的残留检测提供了快速高效的检测手段，由于采用的是多克隆抗体，费用较低并且稳定性和重复性较好，检测限(IC 90 )为0.0126ng/ml、半数抑制量(IC 50 )为0.54ng/ml和检测范围(IC 20 ～IC 80 )为0.04467～14ng/ml。

The membrane protein gene of Infectious Bronchitis Virus like was obtained from alpacas for the first time. And it was cloned into the pMD-18T vector.

本试验首次从羊驼体内获取了禽传染性支气管炎样病毒的膜蛋白基因，并克隆到了pMD-18T载体中，获得了膜蛋白基因的克隆质粒。

T; said billings."in species where it has taken place, there were a lot of abnormalities."

比林斯说，任何灵长类动物克隆都未曾完成，而在已经克隆的种类中，已出现了许多异常现象。

We present a quantum error correction scheme based on quantum cloning. This scheme shows the relationship between quantum cloning and quantum error correction. 4 To avoid decoherence in quantum algorithms, we construct a decoherence-free subspace by using multiple quantum coherences.

该方案一方面说明了量子克隆与量子纠错存在一定程度上的联系，另一方面也反映出一些量子克隆过程本身具有一定的抗消相干的能力。4）提出用二维NMR中的多量子相干实现无消相干子空间，并在实验上验证了该DFS的避错能力。

Identification of susceptibility genes for complex diseases in animal model, here rat arthritis as an example, may include the following steps:①selecting inbred animal strains and establishing disease models;② segregating breeding in F2 animals and running linkage analysis to find QTLs;③ producing a series of congenic strains to narrow down QTL region under help of STR or SNPs markers;④ carrying out positional cloning of susceptibility genes, and ⑤ confirming the function of susceptibility genes.

以大鼠类风湿关节炎模型为例，定位克隆易感基因步骤包括：①选择亲代近交系，建立关节炎模型；②分离育种，连锁分析、确定数量性状位点；③建立Congenic系、窄化QTL区域；④位置克隆基因；⑤易感基因的功能验证。

Before we assume that the market for human clones consists mainly of narcissists who think the world deserves more of them or neo-Nazis who dream of cloning Hitler or crackpots and mavericks and mischief-makers of all kinds, it is worth taking a tour of the marketplace.

我们总是一相情愿地假定克隆人的市场主要由那些认为世界上应该多几个自己的自大狂、梦想克隆希特勒的新纳粹或者是疯子、怪人、惹事鬼构成，但在下这个结论之前，我们很有必要先做一次克隆市场之旅。

Since the first identification and cloning of a SUT cDNA from spinach, many SUT cDNAs have been cloned and characterized in 34 different plant species, in both dicots and monocots.

自从菠菜中克隆到第一个SUT基因以来，已先后有多个SUT基因的cDNA得到克隆与功能分析，涉及34种双子叶与单子叶植物。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。