克隆

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

The growth speed of clone is different in various inoculating density.

在不同接种密度下，克隆的生长速度不同，实验结果表明：分瓶数量越多，克隆总重量增长越多。

Cell suspension was prepared and diluted when diameters of the fourth passaged cell spheres were 2μm.

当第4代细胞大多数克隆球直径约为2μm时，利用有限稀释法进行单细胞克隆培养。

The highest frequency of clonal expanded T cells predominated in Vα12 (3 out of 5 positive samples).

其中，以Vα12亚家族出现克隆性增殖T细胞频率（35例）最高，有2例仅存在单一的克隆性增殖Vα3亚家族T细胞。

Whereas the majority of 29 Vα subfamilies could be found from peripheral blood of 9 cases normal adults.

脐血中TCR Vα亚家族T细胞分布存在倾斜性，绝大部分TCR Vα亚家族T细胞均呈多克隆性，极个别可出现寡克隆性。

This study was purposed to investigate the dynamic change of clonal proliferation of T cell receptor V subfamilies in peripheral blood of patients received allo-hematopoietic stem cell transplantation and to analyze the relationship between T cell clonal proliferative changes and GVHD.

本研究观察异基因造血干细施移植患者外周血T细胞受体Vβ亚家族克隆性增殖的动态变化，分析T细胞的克隆性演变与GVHD的关系。

This study was purposed to investigate the dynamic change of clonal proliferation of T cell receptor V subfamilies in peripheral blood of patients received allohematopoietic stem cell transplantation and to analyze the relationship between T cell clonal proliferative changes and GVHD.

本研究观察异基因造血干细胞移植患者外周血T 细胞受体 Vβ亚家族克隆性增殖的动态变化，分析T细胞的克隆性演变与GVHD的关系。

The clones at the same position were picked, amplified and tittered to continue the next round of screening. One hantavirus candidate receptor clone was obtained after three rounds of selection.

挑取与此位置相对应的克隆，滴定扩增后进行下一轮筛选，共进行了3轮筛选，挑选出一待选汉坦病毒受体克隆。

But on Thursday, the 100-year-old European beech on Central Parks Cherry Hill was the center of attention - chosen by New York city officials as the first of 25 "historical" trees to be cloned as part of a plan announced last year to add a million new trees to streets, parks and public spaces over the next decade.

而本周四，位于樱桃山中央公园的这颗百年欧洲山毛榉树成了众人关注的焦点--它被纽约市政官员选入25棵首批&克隆古树&。&古树克隆&项目是纽约市政府去年公布的百万植树计划的一部分，纽约计划在未来十年内在市内街道、公园及公共场所增加100万颗树木。

EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length of open reading frame and 3'UTR sequence were cloned by RACE technique.

方法］采用简并引物PCR克隆得到黑唇鼠兔LDH-C基因的EST序列，再根据EST序列采用RACE技术克隆出基因的开放阅读框全长，和3'UTR序列。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。