克隆

Acknowledging the potential for his defeat, he also sent a single Spaarti cloning cylinder to his facility on Nirauan to produce a single, unaccelerated clone of himself.

在获悉自己有可能兵败身亡后，他将一个斯帕提克隆罐送至尼劳安，并为自己制造一个未经加速的克隆体。

Results Trastuaumab inhibited cell proliferation, colony formation, and decreased or eliminated the levels of two uncharacterized phospho-proteins （ molar weight about 90 000 and 40 000） in SKBR3 cells shedding high level of HER-2 ECD expression.

结果高HER-2 ECD水平的SKBR3细胞生长及克隆形成率明显被曲妥珠单抗抑制，在相对分子质量为90000和40000左右分别有1条未知磷酸化蛋白明显降低或基本消失，而细胞生长及克隆形成率未受影响的SKOV3细胞中此蛋白无明显变化。

This is one case where even people unnerved by cloning might see it as not the worst option.

从这个例子，即使对克隆技术不感冒的人们也能发现基因克隆不是最坏的选择。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

Fragment as probe to hybridize with more than 30, 000 phage clones of a uamda gtlO library constructed from poiy~+RNA isolated from 48 hours NaCI (200mM) treated leaves from L. sinense. Several potential. phage clones were got and need further character i zat ion.

利用已构建的补血草的λ。gt10 cDNA文库（RNA来源：补血草幼苗用200mM NaCl处理48小时后提取叶片RNA），对三万多个噬菌体克隆分别用用136号片段做探针进行杂交筛选，得到了一些阳性克隆，进一步的鉴定工作仍在进行中。

Full length nucleic acid sequence of recombinant pGEM-AC-7 were determined and we found recombinant pGEM-AC-7 was 1146 bp long, inculding all open reading frame except initiation coden ATG and 192 bp 3-end non-coding reagion.

DNA序列分析和PCR鉴定表明，插入片段均由5&端特异引物单个引物扩增出来，对7号重组克隆插入片段DNA全序列分析表明，7号重组克隆插入片段全长1146 bp，包含了除启始密码子外的全部编码区和192 bp的3&端非编码区。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

Yet while Little Nicky, who was delivered two weeks ago, frolics in his new home, the kitten's creation and sale has reignited fierce ethical and scientific debate over cloning technology, which is rapidly advancing.

这家公司还表示，他们计划在明年5月之前&生产&出世界上第一只克隆狗。但据报道，这种做法已经开始遭到反对动物克隆人士的抗议。

Cultures from environmental surfaces yielded an average of 12.7 colonies with a range of 1 to 80, while cultures from armpits and groins yielded colonies of bacteria "too numerous to count."

周围物品表面的细菌培养形成的克隆数从1至80不等，平均12.7个；而腋窝及腹股沟的细菌培养所产生的克隆数却&数不胜数&。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。