克隆

So thealternative splicing of tra-1 mRNA maybe happened.The whole procedure initiates a novelty technique in the study of Mermithidae. What is more, it has settled a rigid foundation for the further research of the gene in sex detennination.

综上所述，本项研究的创新之处在于首次通过秀丽隐杆线虫基因组用PCR方法克隆tra-1基因的锌指部分序列，不仅发现其内含子序列的组成结构对其可变性剪接有重要的影响，而且本研究结果将对克隆昆虫病原等索科线虫的相关性别决定基因提供参考价值和比较基础。

A phenylalanine ammonia-lyase gene was clone from Nerium oleander for the first time and it was also the first cloned gene involved in anthocyanin metabolic pathway in N.oleander.

首次从夹竹桃中克隆得到苯丙氨酸解氨酶基因cDNA片段，并命名为NoPAL1，GenBank登录号为AY747677，这也是首次从夹竹桃花青苷代谢途径中克隆得到的基因。

Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.

方法根据Eg95基因序列设计引物，用PCR方法分别从新疆株细粒棘球绦虫3个不同发育阶段所构建的cDNA文库中克隆获得Eg95目的基因，将其克隆至pUCm-T载体、测序并进行序列分析。

Therefore the concept of reverse cloning technique was proposed based on ooplasmic transfer.

以卵胞质转移为基础的&逆向克隆技术&是否能达到克隆动物的结果前景喜人。

Inclusion criteria: The articles related to the research of ooplasmic transfer, cloning or reverse cloning.

纳入标准：文章所述内容应与卵胞质移植、克隆或逆向克隆研究相关。

Total 18 crenarchaeal sequences were obtained and divided into 12 OTUs. The average similarities between the clone sequences from Wuming hot spring and Rebao hot spring and their closest sequences deposited in GenBank is 92.56% and 93%, respec-tively.

一共得到 18 个泉古菌克隆序列，可分为 12 个OTUs，两温泉的克隆序列与已知GenBank上关系最近序列的平均相似性较低，无名泉为 92.56%，热爆区为 93%。

Results A total of 121 positive clones were randomly screened from the library and 22 Operational Taxonomic Units were determined. BLAST analysis indicated that all OTUs were affiliated with the phylum Crenarchaeota.

从冷泉沉积物古菌16S rRNA 基因文库中随机挑选了121个阳性克隆，共得到22个不同的可操作分类单元，BLAST结果表明全部克隆子归属于泉古菌门中免培养类群。

The result showed 41 clones distribute in 26 OTUs, which exist in Acidimicrobidae and 7 suborders of Actinobacteridae respectively, especially the dominant community of Yutian pit was streptosporangineae instead of streptomycete, which accounting for 42.3% of sequenced clones.

结果表明， 41个克隆序列属于26个OTUs，分别分布于放线菌门放线菌亚纲的7个亚目和酸微菌亚纲，其中链孢囊菌亚目(Streptosporangi- neae)中放线菌组成丰富，占到了全部挑选克隆的42.3%，是于田盐池放线菌群落中的优势菌，而链霉菌不是高盐环境放线菌的优势菌群。

Bama Mini-pig cloned embryos of 0.5-1.5 days old were surgically transferred to the oviductal ampulla of two female Bama Mini-pigs and two female Guangxi Luchuan pigs, of which the two female mini-pigs and one Luchuan pig returned to estrus, and one female Luchuan pig gave birth of one male cloned Bama mini-piglet.

利用刺入式手术胚胎移植法，将0.5~1.5日龄巴马小型猪克隆胚移植到2头巴马小型猪和2头陆川猪的输卵管壶腹部。其中2头巴马小型猪和1头陆川猪返情，另外一头陆川猪于2007年10月13日产下1头克隆雄性巴马小型猪。

Aims In natural environments, resources of critical importance to plant growth are patchily distributed, even at a small spatial scale. The aims of this study are to address: 1 Centella asiatica exploitation of heterogeneous microsites in different habitats, 2 hierarchical effects of habitats and microsites with different herbaceous coverage on growth traits of C.

为研究克隆植物在不同生境中对小生境的利用能力和对不同尺度异质性生境的等级可塑性及其格局差异，比较了林缘和荒草坡不同草本层盖度小生境中积雪草分株种群、克隆片段和叶水平的形态和生理特征。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐