克隆

Also studied was aza Diels-Alder reaction catalyzed by polyclonal antibody. The bicyclic [2, 2, 2] heptene and the aimed immunogen were synthesized via the key intermolecular asymmetric aza Diels-Alder reaction. Then four polyclonal antibodies were obtained by immunizing rabbit.

在研究多克隆抗体催化氮杂Diels-Alder反应中，通过分子间不对称氮杂Diels—Alder反应为关键反应合成了双环[2，2，2]庚烯类半抗原和目标抗原；用目标抗原免疫动物兔后得到了4株多克隆抗体，经动力学测试表明：多抗Aza-Bsa-3#能催化氮杂Diels—Alder反应的进行。

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

Tibet is a center of distribution and differentiation of genus Hordeum in China, and has a great deal of species resource. The sequences of the nontranscribed intergenic spacer region of 5S nuclear ribosomal DNA was studied in 18 varieties of the wild close relative of barley from different geographical region from Tibet of China and Turkmenistan.

选取来自西藏不同地理区域的17份近缘野生大麦材料和1份国外的近缘野生大麦材料，利用直接PCR、T-载体转化克隆、克隆产物测序的方法获得了它们的5S核糖体DNA NTS（Nontranscribed intergenic spacer）序列。

Based on the degenerated primer, the cDNA of open reading frame of growth horme gene for black carp and Japanese crucian carp were cloned and sequenced through RT-PCR, which were used for the"all-fish"transgene construction and preparation of polyclonal antibody against GH for black carp.

基于鲤科鱼类生长激素基因序列，通过RT-PCR技术，克隆了青鱼、日本白鲫生长激素基因编码区cDNA序列，以此来构建&全鱼&基因载体和研制生长激素多克隆抗体。

Based on the clonal selection principle and idiotypic immune network theory of biological immune system,a dynamic clonal adjustment strategyis proposed.

基于生物免疫的克隆选择和独特型免疫网络机理，提出了动态克隆调节策略。

Based on the clonal selection principle and idiotypic immune network theory exhibited in biological immune system, a new immune algorithm-the clonal selection adjustment algorithm is proposed.

基于生物免疫系统克隆选择机理和独特型免疫网络理论，提出了一种新的免疫算法―克隆选择调节算法。

Based on the clonal selection algorithm, with the combination of the dynamic adjustment idea between antibodies of the idiotypic immune network theory, a new immune algorithm, the clonal selection adjustment algorithm, is proposed and applied to the design of IIR digital filter.

在克隆选择算法的基础上，结合独特型免疫网络理论中抗体间的自适应动态调节思想，提出一种新的免疫算法―克隆选择调节算法，并用于IIR数字滤波器的设计。

Methods: Clone the genes of ScFv (OC183B2) and PE38 into PET30a and then express the immunotoxin after induced with IPTG.

采用基因工程技术，经过3次亚克隆，将编码单链抗体的基因及编码毒素的基因亚克隆至原核表达载体pET30a上，并在IPTG的诱导下进行表达。

The distribution of PTA1 molecule on NK cell, NK cell lines and NK cell clone was detected by indirected immunofluorescence staining and FCM analysis. The results showed that PTA1 expression rate on activated NK cell in MLC was above 60%.

结果证明这种方法能够稳定获得NK细胞克隆，应用流式细胞仪检测获得的一株NK细胞克隆表型为 CD56CD3－。

The melanotic phenomena mediated by PPO is widespread in both mosquitoes and other insects that being involved in sclerotization and canning of the egg shell and cuticle,wound healing and encapsulation of invading microorganisms.

目前，已从冈比亚按蚊克隆到6种不同的PPO基因，但斯氏按蚊与库态按蚊仅克隆到一种，并对其在按蚊抗疟原虫感染中的作用进行了初步研究。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐