克隆

Coli JM109.The rabbit anti-z66 polyclonal antibody was prepared successfully. Conclusion: This study was a foundation to research the mechanism underlying the the unidirectional flagellar phase variation of H:z66 positive Salmonella enterica serover Typhi.

成功克隆表达了H：z66阳性伤寒沙门菌鞭毛素基因fljB：z66，并制备了相应的多克隆抗体，为今后深入研究H：z66阳性伤寒沙门菌的单向相变换机制奠定了基础。

In this research, FvndhB gene segment was obtained, and the phylogenetic analysis was researched. It established basis on cloning the span of FvndhB and studying the characteristics of FvndhB gene expression in Fraxinus velutina Torr.

本研究首次从绒毛白蜡中克隆了ndhB基因，并对其进行了同源性分析，为进一步克隆绒毛白蜡ndhB全长基因及分析其表达特性奠定了基础。

Coli and S.aureus , the experiment showed that recombined gene peptide has the antibacterialactivities.Conclusion The recombined gene antibacterial peptide was expressed successfully in the fusional pTYB vetor and the fusion gene peptide CA(1 ~8-MA(l ~12),CAM has the antibacterial activities to E. coli and S.aurens. While how to frame a more efficient expression system should be studied further , and its other biological activities are still to be tested.

本实验通过基因克隆方法拼接重组基因抗菌肽与融合表达载体pTYB12并在大肠杆菌中进行表达成功，抑菌活性的检测结果表明重抗菌肤的克隆表达与活性初步测定中文摘要组基因抗菌肤CA(1一8卜MA(1一12)，cAM〕对大肠杆菌与金黄色葡萄球菌均有一定的抗菌活性，如何进一步提高抗菌肤的表达量和有关重组基因抗菌肤的生物学活性还有待于进一步研究。

The gene cloned from gibel carp is named Carassius auratus gibelio hira and that from coloreded crucian carp is named Carassius auratus hira .

从银鲫中新克隆的基因被命名为Carassius auratus gibelio hira，从彩鲫中新克隆的基因被命名为Carassius auratus hira。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

Result: the regions with strong to very stong labeling by the GDNF probes were as follows: the caudate-putamen, the habenular nucleuse, and some areas of the brainstem and spinal cord, the trigeminal ganglion, the dorsal root nerve ganglion.

本实验利用原位杂交技术结合RT-PCR技术观察了GDNF从E16～成年大鼠神经系统内的分布；为了更进一步研究GDNF在神经发育中的作用与作用机制，本实验还利用基因克隆技术克隆了人GDNF基因并进行了原核表达GDNF蛋白的研究。

Objective Chronic myeloid leukemia, with of chromosome, is one of primary clonal malignancies of hemapoietic stem cell. Philadelphia chromosome is the hallmark of malignancies clone in CML.

研究背景与目的：慢性髓系白血病是一种原发于多能造血干细胞的恶性克隆性疾病，第9号与第22号染色体相互易位形成Ph染色体是其肿瘤恶性克隆性标志。

To investigate the clonal rearrangements of immunoglobulin heavy chain gene and T-cell receptor γ gene in peripheral blood cells of patients with hemapoietic malignancies and their significance, the clonal rearrangements of IgH and TCRγ genes were detected by polymerase chain reaction in peripheral blood cells of patients with hemapoietic malignancies, including 32 cases of non-Hodgkin lymphoma, 18 cases of acute myeloid leukemia, 24 cases of multiple myeloma, 8 cases of acute lymphoblastic leukemia and 6 cases of chronic lymphoblastic leukemia.

检测各种血液系统肿瘤患者外周血细胞免疫球蛋白重链基因和T细胞受体γ基因克隆性重排并探讨其意义。通过多聚酶链式反应方法检测32例非霍奇金淋巴瘤、18例急性髓性白血病、24例多发性骨髓瘤、8例急性淋巴细胞白血病及6例慢性淋巴细胞白血病患者外周血细胞IgH及TCRγ克隆性基因重排。

TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction were cloned using TA easy vector. 3-5 clones selected from each patient randomly were sequenced, and then compared with TTV DNA sequences in GenBank.

对6例血友病人血清中分离的TTV进行基因克隆，从每例病人的标本随机选多个克隆进行测序，比较其核苷酸和氨基酸序列。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐