克隆

By blast in GenBank, clone Y1218 shared 72% homology with Chromobacterium violaceum, clone Y1814 shared 88% homology with Pseudomonas putida,clone Y0140, 80% with Bacillus sp., and clone Y1038, 71% with Pseudomonas fluorescens. Some clones had homology with the degreadation of aniline, methyl parathion microbial organisms.

有80%的同源性，Y1038克隆和荧光假单孢有71%的同源性，有部分克隆和一些具有降解苯胺、甲基对硫磷等有机物的微生物有同源性。

Through analyzing static clonal selection algorithm, this thesis proposes a multilayer dynamic clonal selection algorithm.

本文通过对静态克隆选择算法的分析提出了一个多层动态克隆选择算法。

Results In the clonogenic assay group, the detected surviving tumors had a clonic function. There was no difference of radiosensitivities between Colo-205 cells living in suspension and on adherence.

结果 在克隆测定组，被检测的活存细胞均具有克隆能力；Colo 205悬浮生长和贴壁生长细胞对放射线的敏感性没有差别。

YIp5 plasmid which is replicable in E. coli but not in yeast is served as clonic carrier and ARS sequence in total DNA of rice cloned by air gun experiment.

以能在大肠杆菌复制，但不能在酵母菌中复制的YIp5质粒作为克隆载体，鸟枪法克隆了水稻总DNA中的ARS顺序。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The results showed that the clonal reproduction was preponderant, but it has still a high genetic diversity (Ho=0.7148). At the same time, Corylus heterophylla Fisch genetic individual was found. A single cluster was basically a genetic individual; adjacent clusters were basically the same genotype and clonal reproduction has larger percentage.

结果表明：平榛虽然克隆繁殖占有优势，但仍具有较高的遗传多样性(Ho=0.7148)；同时确定了平榛的遗传个体，同1株丛内个体基本上为1个独立的遗传个体，相邻的株丛个体基因型基本一致，克隆繁殖比率较大。

The crocin synthesis mechanism was investigated at the molecular level by studying the genes related to crocin syntheses.

对藏红花苷合成的相关基因进行了初步分离与克隆实验研究，旨在分离与藏红花苷合成相关的特异性表达的cDNA克隆，为进一步从分子水平上研究藏红花素的合成机理奠定基础。

Phycobiliprotein are important light-harvesting pigment proteins in Cyanophyceae, Rhodophyceae, Cryptophyceae and a little Pyrrophyceae.

中文题名极大螺旋藻藻蓝蛋白基因的克隆及其表达研究副题名外文题名 Cloning and sequencing of the phycocyanin gene from Spirulina maxima and its over-expression in Pichia pastoris 论文作者于平导师岑沛霖学科专业生物化工研究领域\研究方向学位级别博士学位授予单位浙江大学学位授予日期2003 论文页码总数110页关键词藻胆蛋白基因克隆藻蓝蛋白基因螺旋藻馆藏号BSLW /2003 /Q949 /32 藻胆蛋白是蓝藻、红藻、隐藻和一些少数甲藻中的一种重要的捕光色素蛋白。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐