克隆

To clone new disease gene of CMT, many myelin and myelin-related gene had been analysised by homologue searching. Quaking mouse, one of the mutation mouse with the clinic feature similar to Trembler mouse (the gene mutation mouse of PMP22) with the characterization of demylination in both the central and the peripheral nervous system, its disease gene had just been cloned accession No.

为进一步克隆新的CMT致病基因，在分析出CMT已克隆的基因均为与髓鞘有关的基因后，通过筛选有关髓鞘基因，我们发现，quaking基因突变小鼠因髓鞘形成障碍而出现震颤，其中枢和周围神经均有脱髓鞘，类似于PMP22基因突变鼠"Trembler"的临床表现。

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

Methods the gene of glutathione-dependent peroxidase in trypanosome cruzi was cloned and expressed and the genome of t.cruzi was anlayzed.

使用基因克隆与表达鉴定的方法学对克氏锥虫谷胱甘肽依赖的过氧化物酶基因克隆和表达。

In the opinion of the British source, ATI will releasefor the market only video card with 1 Gb of memory type GDDR-4, support GDDR- E will remain the uncalled-for function of the chip r600, or it will be stored for the low-end solutions.

在民意的英国来源保险市场将释放为视频卡仅1绿化记忆型克隆-4支持克隆娥仍将不必要的功能晶片r600，否则将被储存的低端解决方案。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

There are predominant clone groups among Vibrio cholera strains of different periods and districts, as well as different clone groups.

不同年代和地区之间流行的霍乱弧菌存在一定的优势克隆群，但也存在不同的克隆系。

This work would lay the foundation for further study on regulating and expressing mechanism of MyoG in Gansu wapiti.

为此，本研究对甘肃马鹿MyoG基因的启动子区进行了克隆和生物信息学分析，以期为鹿MyoG基因全长序列的克隆、结构特征、表达调控机制及其突变与鹿产肉性能等方面的研究提供基础资料。

I'm sorry, but I'm not going to watch the Clone Wars TV series until I've seen the Clone Wars movie.

抱歉，可在看电影版《克隆战争》之前，我是不会去看电视剧版《克隆战争》的。

In addition, it has been found from several marine biologies such as zebra fish, trout arid crab. But such research taking sea perch as the material has not been done in the world.

而海洋经济鱼类如鲈鱼，却从未被克隆与研究，本实验就以海洋经济鱼类鲈鱼作为实验材料，对Syntaxin 1B基因进行克隆和序列分析。

More clones expressed phenotypes of osteoblasts (48%), chondrocytes (43%), and neuron-like cells (52%) than of adipocytes (12%), possibly due to the loss of adipogenic ability after repeated subcultures.

多数克隆的表型是成骨细胞（48%）、软骨细胞（43%）、神经元样细胞（52%），而脂肪细胞表型的克隆仅12%，这可能由于多次传代培养后成脂能力的丧失。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐