克隆

The 6 AChE Genes from L.bostrychophila,L.entomophila and L.decolor were demarcated into Insect TypeⅠAChE Gene and Insect TypeⅡAChE Gene.4 Gene cloning of nAChR and mRNA expression level in L.bostrychophilaTwo nicotinic acetylcholine receptor subunit genes,Lb al and Lb a8,were cloned from the psocid L.bostrychophila.The full length cDNAs of Lb a1(GenBank Accession No.: EU871527) and Lb a8GenBank Accession No.

昆虫的两类AChE严格的区分，其中Ⅰ型AChE先与脊椎动物AChE聚合后才与Ⅱ型AChE相聚，说明两类AChE基因在物种分化前就已经分化。4嗜卷书虱烟碱型乙酰胆碱受体基因克隆及其mRNA表达水平研究利用RT-PCR和RACE技术成功克隆获得了嗜卷书虱2个烟碱型乙酰胆碱受体亚基的全长序列，分别命名为Lb a1(GenBank登录号：EU871527)和Lb a8(GenBank登录号：EU871526)。

Cloning technology is used to multiply valuable gene, for example, in medicine, it is through "cloning" technology produces insulin therapy for diabetes patients, that pygmyism again the high growth hormone and resistant to infection of the interferon, etc.

克隆技术还可用来大量繁殖有价值的基因，例如，在医学方面，人们正是通过"克隆"技术生产出治疗糖尿病的胰岛素、使侏儒症患者重新长高的生长激素和能抗多种病毒感染的干扰素，等等。

The best conditions were: ethanol/acetone as solvent, molecular ratio of racemate to resolving agent 1.0:1.0, using heating as method of reaction, concentration of racemate 20mg/ml, crystallization temperature 1℃, crystallization time 8 days.

最佳结果为：乙醇／丙酮一 5.85，则为溶剂；L＜一卜苹果酸与佐匹克隆摩尔投料比为10二0；佐匹克隆20mg／ml；反应物溶解方式为加热处理；结晶温度l℃；结晶时间sd进行拆分。

Colony formation also displayed the same conclusion. All above results suggest that HPIP is radiosensitive.

克隆形成实验进一步验证了这一结果，辐射后HPIP导致细胞的克隆形成率大幅度下降，说明辐射后HPIP使细胞的生存能力降低，即HPIP具有辐射增敏作用。

Protein expression was examined by using Western????blot. Results: The Fas mRNA and P53 mRNA expression was irrelevant to the radiosensitive heterogeneity of NPC; the Bcl????

2蛋白表达。结果：两鼻咽癌亚克隆株Fas mRNA 、P53 mRNA的表达与鼻咽癌放射敏感性异质性相关性不明显；两鼻咽癌亚克隆株Bcl？？？？

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

It has already become the important subject for the scientific circle of cotton in our country that how to occupy a space in competing of cotton study in the future and promote its genomics research.

而采用我国骨干棉花品种、构建具有我国自主知识产权的BAC文库，利用国内外已有分子标记探针钓取和分析含有重要性状的BAC克隆，是发掘分子标记、克隆重要基因、深入开展棉花功能和比较基因组学研究的基础和关键。

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别，采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞，经7天G418(600μg／ml)药物筛选，共获得30个neo阳性细胞克隆，其中12个为非绿色细胞克隆，仅有7个可以扩大培养。

Oligoclonal expansion T cells could be detected and Vβ21 oligoclonal T cells were identified in all 3 cases.Conclusions The skew distribution and clonal expansion of TCR Vβ subfamily T cells could be found on the patients with CML.

结果 3例病人仅存在3～9个TCR Vβ亚家族T细胞，部分Vβ亚家族T细胞呈寡克隆性增殖，Vβ21的寡克隆T细胞见于全部的3例病人中。

One hundred and eighty-four cDNA clones were identified as submergence specifically induced or highly expressed.

对其中155个cDNA克隆测序并去除重复克隆后，共得到95个差异表达的cDNA片段。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐