克隆

A novel Multi-Objective Immune Algorithm based on Memory Clonal Selection is proposed in this paper by introducing the mechanism of clonal selection in organismal immune system.

借鉴生物免疫原理中克隆选择机理，设计了一种基于记忆克隆选择的多目标免疫算法。

One positive clone that can integrate specifically with ouabain with higher affnity was sequenced.

选取一个与特异性抗原结合活性比较高的噬菌体抗体阳性克隆，将其V区基因克隆后进行序列分析。

Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology.

设计并合成siRNA，脂质体siNEO FX转染Hela细胞，分别用MTT法，双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢，软琼脂克隆形成率降低，G0/G1，期细胞比率增加。

We report here creativity using compound solvent as solvent is the unique of the new technology on the basic of perpendicularity experiments and with the actual situation of our country, the effect of reaction temperature, reaction time, rate of material were optimized. Rate of yield can reach beyond 98% and this technology has successfully applied in practical production with the output of 300t per year.

解决以上两个问题是改进得克隆生产工艺的关键，本文针对上述问题，并且结合我国实际情况，在正交试验的基础上创造性的选择了复合溶剂，改进了得克隆的生产工艺，确定了最佳的投料比、反应温度、反应时间等反应条件，溶剂可以循环利用，并且得到了98％以上的高产率。

The results showed that the 20 phagocyte clones, which was selected at random after three times selection, could combine with BmVUB with ELISA analysis.

结果表明，3轮筛选后随机挑取20个噬菌体克隆，ELISA 鉴定表明20个克隆均可与BmVUB结合。

The further identification indicated that free BmVUB could block the combination of 10 positive phagocyte clone with solid BmVUB.

对这10个阳性噬菌体克隆进一步鉴定，游离BmVUB可阻断10个阳性噬菌体克隆与固相化BmVUB结合。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

On the one hand, there are many serious problems on moral,ethic,society and law appearing with procreative clone.

治疗性克隆不会像生殖性克隆那样产生严重的道德、伦理、社会或法律问题。

Compared with known protamine gene sequence, there are three single nucleic acid variations in our senquence and their homology is up to 97.1%.

本文以pUCT为克隆载体克隆到了鱼精蛋白编码基因，与已知序列相比较，只存在3处变异，同源性高达97.1%。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐