克隆

Cloning and characterization of novel genes involved in cell growth and prolifereation using the strategy of homology screening;;Cloning,chromosome mapping and tissues expression patterns of NQO3A2 and human hereditary methemoglobinemia gene

中文题名应用同源筛选法分离与克隆细胞生长、增殖相关基因的研究；人类NQO3A2 cDNA的克隆、定位，及其与NQO3A1表达谱的比较分析

The cloned cDNA fragment in sugarcane had the same conserved amino acid sequences for GA20-oxidase as those in other monocotyledon plants, and shared respective homologies of more than 80% and more than 75% in the nucleotide and amino acid sequences with many other monocotyledon plants.

通过RT-PCR扩增得出1条约740 bp大小的DNA片段，将其克隆至pMD18-T载体上，而后对重组克隆进行测序，结果表明所获得的甘蔗GA20氧化酶基因片段由740碱基组成，编码246个氨基酸。

In a whole, the genetic diversity of clonal plants is much higher than that had been expected before. Although the genetic diversity is lower than their sexual congener, widely genetic monomorphism has been rarely found.

总体而言，克隆植物拥有比早期推测大得多的遗传变异，虽然克隆种与其近缘有性繁殖种相比，遗传多样性较低，但广泛的遗传单态性却很罕见。

By the test of clonal growth in soft agar medium , we compared the ability of cloning and proliferating among these three kinds of cells. The cell cycle was analyzed by FCM and morphosis of cells was observed under transmission eletron microscope.

通过细胞生长曲线，比较卵巢癌细胞系HO－8910与8910－p93、8910一pCDNA3.1（转染空载体ncDNA3.l）的增殖情况；通过软琼脂克隆形成试验比较这3种细胞的克隆形成能力和增殖能力；利用流式细胞仪检测这3种细胞的细胞周期的改变情况：利用透射电镜观察转染p93基因的细胞的超微结构变化；利用裸鼠移植瘤实验比较HO七与8910个93在动物体内生长状况。

X are under implementation, there is a need for hard drive cloning, please enter the Ghost subdirectory, run Ghost.exe procedures should be noted that, if it is in The program runs under DOS when running the program to start before the best mouse driver for DOS, because the operation of Ghost is like a window screen frame, use the mouse to click to choose will be more convenient - although it can be used to operate the keyboard.

Ghost就是克隆硬盘的程序，该程序在DOS下、Windows9.X下都可执行，所以要进行硬盘的克隆，请先进入到Ghost子目录，运行Ghost.exe程序，需要注意的是，如果是在DOS下运行该程序时，在运行该程序前最好启动DOS的鼠标驱动程序，因为Ghost的操作画面是仿窗口画面，使用鼠标点击来选择会方便一些--虽然也可以用键盘来操作。

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因，分别与克隆载体pMD18-T连接后，转化感受态细胞，对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序，结果表明，E05株六邻体蛋白基因长度为2733 bp，共编码910个氨基酸。

Objective To express human myoglobin and prepare its polyclonal antibody.

目的 克隆、表达人肌红蛋白基因，并制备人肌红蛋白多克隆抗体。

This paper proposes a clonal selection algorithm with negative selec- tion operator and genetic operator .

本文提出了一种含有否定选择算子和遗传算子的克隆选择算法，通过克隆选择算法产生多样子代检测器，并且从中选择比其父代更优的检测器去取代父代检测器，这样一代一代循环，使检测系统具有更好的覆盖空间。

Objective To study the relationship between DNA fragments in ENV gene of SIVmac and viral replicating ability. Methods Reciprocal recombinants were constructed by replacing the clonable DNA fragments of ENV region of the nonpathogenic SIVmac142 clone with the pathogenic SIVmac 239 clone.

猴免疫缺陷病毒(simian immunodeficiency viruses SIV)是一组在致病性和毒力方面具有多样性的灵长类动物病毒，其中，SIVmac239克隆感染的猴表现为与人类AIDS相似的疾病，称之为致病株，而SIVmac142克隆仅引起猴发生病毒血症，不发生临床综合征，称之为非致病株〔1〕。

The efficiency of the nuclear transfer was 63.6%(63/99).Forty-seven clone embryos were transferred into 5 recipient goats. After embryo transfer, two recipient goats have not been seen oestrous within 2 oestrum, and one of the two recipient goats was examined positive by gravid testing, which testified the clone embryos were viable in the uterus of recipient goats.

阳性细胞克隆中的2株(M9，M10)已经进行了首次细胞核移植，核移植成功率为63.6％(63／99)，将47个发育较好的克隆胚胎进行了胚胎移植，共用受体羊5只，2只受体羊2个情期未见返情，在没有返情的2只受体山羊中有1只妊娠检测阳性，说明转基因克隆胚胚胎移植后在受体山羊体内发育良好。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐