克隆

Gnomic fragments of 4～20 kilobases were obtained by digesting genomic DNA of V.cholerae O 139 with restriction endonuclease Eco RI,then plasmid gene bank was constructed.

随机筛选重组克隆，获得一株可与O1 39霍乱弧菌抗血清发生凝集反应的重组克隆，命名为大肠杆菌DH5α(pMG32 0 )。

It had come as quite a shock to find yet another Zor-clone waiting on Tirol after she had just finished her brief go-round with one on Earth.

当她结束了自己与佐尔的一个克隆体在地球上的短暂的遭遇后来到泰洛的时候，却非常震惊的发现了另外一个佐尔的克隆体。

Aiming at the two major migrating routs of wild birds in China, the ecological distribution and prevalence of avian influenza were studied based on the investigation and analysis of the occurring and relative influential factors of highly pathogenic avian influenza, serology of free birds of HAPI areas were investigation and analysis, live stocks and poultries of some areas of Heilongjiang province were investigation and analysis, the HA gene of avian influenza virus A/Goose/XJYL/10/2003HA(H_5N_1)isolated from Xinjiang goose were cloned and sequenced, NS gene of avian influenza viruses (H_5N_1) isolated in Xinjiang were also cloned and sequenced.

本文在调查、分析禽流感发生情况以及影响疫情相关因素的基础上，针对我国野鸟迁徙的2条主要路线，对我国禽流感病毒生态分布和禽流感流行现状的研究，对爆发高致病禽流感疫区的自主活动鸟类禽流感的血清学调查与分析，对黑龙江省部分地区散养畜禽流感病毒感染的血清学调查与分析，并对鹅源H5N1亚型禽流感病毒新疆分离株A／Goose／XJYL／10／2003HA基因的克隆和序列测定与分析，对H5N1亚型禽流感病毒新疆株NS基因的克隆和序列分析。

Cloned leukemic cells can proliferate in quantity and accumulate in marrow and the other blood-cell forming tissues,and also infiltrate in other organs and tissues,which will inhibit the normal hemopoiesis.

白血病是一类造血干细胞的克隆性疾病，克隆的白血病细胞在骨髓和其他造血组织中大量增生积聚，并浸润其他器官和组织，而使正常造血受抑制。

Results: A different expression cDNA subtractive bank was successfully established from the human nasopharyngeal carcinoma cell line CNE2Z; Six cDNA clones were highly expressed in the lower metastatic strain CNE2ZH5; aligned to sequences of the GenBank database by BLASTN, the six cDNA fragments were found to homologize highly with known human genes.

结果：成功构建人鼻咽癌细胞克隆株差异表达cDNA消减文库，从随机测序10个克隆中获得6个在低转移鼻咽癌细胞株中高表达cDNA序列，它们都与已知的人类基因片段具有高度同源性。

On the basis of our results,IFN-θnot only has the sequence homology to other typeⅠinterferons,but also matches the primary properties of typeⅠinterferons in antiviral, IFNαR receptor binding and virus inducing,etc.

首先，将PoIFN-α基因克隆入pcDNA3真核表达载体中，筛选阳性克隆后大量抽提质粒，通过脱水化-再水化步骤将重组质粒包裹入阳离子脂质体内制备成核酸佐剂(IFN-adjuvant)。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

A clear and inarguable demonstration of an open-source application achieving success by being better, and being better by being different. Had FF been an IE clone, it would have vanished into obscurity. And had Linux been a Windows clone, the same would have happened.

这是一个开源软件通过自身改进取得成功的清晰的，不可争辩的例子，而这种改进正是源自于它不同于IE，FF克隆IE这种说法已经永远的消失了，同样的linux克隆windows的说法也将一去不复返。

In this paper,the relations of micronaire value and the fineness indexes of fibers were theoretically analyzed,including diameter,linear density and maturity.

文章对马克隆值和纤维细度指标之间的关系进行了理论分析，包括马克隆值与纤维的直径、线密度以及成熟度的关系。

Both the interactions of haplotype vs. food (R0: P=0.038; rm: P=0.027) and that of haplotype vs. temperature (R0: P=0.006; rm: P=0.000) had obvious effect on growth reponses of the clonal groups. Moreover, there was significant interaction of haplotype vs. temperature (lorica length: P=0.033; lorica width: P=0.000) and haplotype vs. temperature (lorica length: P=0.027; lorica width: P=0.000) on body size of the clonal group.

生命表实验结果显示，单倍型与食物浓度的交互作用（R0：P=0.038；rm：P=0.027）以及单倍型与温度的交互作用（R0：P=0.006；rm：P=0.000）对种群增长均有显著影响，这4个单倍型克隆群对环境条件具有不同的偏好，并且各个克隆群对于环境变化有不同的生殖反应。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐