克隆

Moreover,about 12.9% transgenic plants appeared stolen green segregation in seedling stage such as etiolation and albino. Among them,about 71% stolen-green seedlings largely died before the period of three-leaf;while about 29% clone largely turned green later period.

另外，还约有12.9％的转基因后代在苗期出现失绿分离，其中约有71％的克隆其失绿苗在三叶期前大部分死亡；约有29％的克隆，其失绿苗后期大部分转绿。

At the very earliest stage, people too are clones- clones of the fertilized egg .

在最初阶段，人也是克隆体—受精卵的克隆体。

This was the first report on the phylogenetic characterization of the symbiotic bacteria associated with the symbiotic flagellate Pyrsonympha vertens in the gut of Reticulitermes flavipes.

根据测序克隆的RFLP图谱所代表的克隆数，说明"白蚁菌群1"和CFB phylum的拟杆菌均为与鞭毛虫P.vertens相关的优势共生细菌类群。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

This paper expounded the situation of animal somatic cell nuclear transfer and biological functions of centrosome and analyzed the inheritance mechanism of centrosome during gametogenesis and fertilization.

文章在分析哺乳动物体细胞克隆存在的问题和简介中心体结构功能的基础上，综述了中心体在配子和受精卵发育过程中的遗传机制，同时阐述了体细胞克隆胚胎中心体及其相关蛋白的研究现状。

Light plays very important role in the growth of Gametophyte clone. Light time and intensity affect its growth directly.

光照对海带配子体克隆的生长起着至关重要的作用，光照时间和光照强度直接影响着克隆细胞的生长。

The growth and dynamics of daughter ramets are affected by genet though clonal integration,and competition from shrubs and herbage of surroundings.

克隆分株的生长、数量动态主要受克隆整合的内部调节机制和外部环境中灌木层、草本层竞争的影响。

The fear was that some rogue scientist would take the work a step further, gestate the embryo in a woman's womb, and make the birth announcement that no one wanted to hear.

他们担心一些居心不良的科学研究工作者会得寸进尺，在女性的子宫中孕育克隆胚胎，从而宣布克隆人类的诞生，这是没有人愿意听到的。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐