克隆

There are many methods for the cloning of novel genes, but one of the most efficient methods is based on the diff...

新基因克隆的方法很多，利用细胞间基因表达的差异是克隆功能性新基因最有效的手段之一。

METHODS: Subtractive cDNA DNA library of PDLF was constructed with a recently developed gene cloning technique that is based on PCR and subtractive hybridization and then screened by restriction analysis and reverse southern dolt blot.

采用基于PCR和消减杂交的基因克隆技术构建体外培养的人PDLF与GF差异表达基因的扣除文库，采用酶切和反向杂交的方法筛选目的克隆，并进行测序和计算机分析。

The wave-particle duality and uncertainty relation in quantum mechanics are very hard to imagine,but they are very similar to the phenomenon of the society and human in which copy and cloning is possible.If the quantum state has something like gene,it is possible to cloning a quantum state.

量子力学中的波粒二象性、不确定性关系等等难于理解，但是却与人类社会中的许多现象绝妙地类似，从更加微观的角度分析，如果量子态也存在类似基因的成分，可以绕开量子不可克隆定理的证明中假定的情况进行克隆。

It possibly brings ethics crisis below.Clone violates therule of natural procreation,induces the confusion of human relations and impacts the form ofphilosophy;the risk and safe coefficient of clone itself can bring up problem when it is usedincorrectly;whether clone interferes nature egregiously and plays the role of God.

它所可能造成的伦理危机主要有：违反自然生殖规律而导致人伦关系的混乱并进而对人生观形成冲击；克隆技术本身的风险与安全系数，并可能由于被不正当使用而引发问题；以及克隆技术是否过份干预了自然，从而扮演了上帝的角色。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Cases of eosinophilia were collected and chromosome specimens of bone marrow cells were prepared by 24hour culture, and Gbanding technique was used for karyotyping.

为探讨染色体异常克隆在嗜酸性粒细胞增多症诊断和鉴别诊断中的意义及克隆性嗜酸性粒细胞增多症涉及的染色体异常，收集了65例嗜酸性粒细胞增多患者的骨髓标本，培养24小时，采用G显带进行核型分析。

The aim of study was to investigate the importance of chromosome aberration in differential diagnosis of eosinophilia and the chromosomal aberrations involved in patients with clonal eosinophilia. 65 cases of eosinophilia were collected and chromosome specimens of bone marrow cells were prepared by 24hour culture, and Gbanding technique was used for karyotyping.

为探讨染色体异常克隆在嗜酸性粒细胞增多症诊断和鉴别诊断中的意义及克隆性嗜酸性粒细胞增多症涉及的染色体异常，收集了65例嗜酸性粒细胞增多患者的骨髓标本，培养24小时，采用G显带进行核型分析。

On the basis of the previous work, we make further study on the mechanism of the denucleation process, especially on the role of different cytoskeletal elements in the denucleation process. To clone and sequence a gene related to terminal differentiation of erythroblast, and construction of two cDNA libraries of erythroblast differentiation. The results of our study are as follows

本文在我室前期工作的基础上，对红系细胞终末分化中的排核机理作了进一步的探讨，克隆、测序并表达了一个与红系细胞终末分化相关的基因，另外，还成功地构建了小鼠终末分化和差减cDNA两个红系分化cDNA文库，为本室以后的基因克隆工作打下了基础。

The eminent University of Chicago ethicist Leon Kass has argued that human cloning would be offensive in part because the clone would "not be fully a surprise to the world."

芝加哥大学知名伦理学家利昂·卡斯认为，人类克隆也许是冒犯，部分原因是克隆"对这个世界来说并不完全是一件意外的事情。"

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。