光度计的

But, because of the complexity of the dye molecule, there has been short of detailed comprehension in its photocatalytic degradation process. The key problem is difficult to detect the intermediates clearly. In this paper, the photocatalytic degradation of azo dye Congo red in water solution was investigated on UV irradiating TiO2 in the homemade reactor. The optimization conditions were determined and then the rate of decolorization, COD decrease, yield of CO2 and varies pH of aqueous solution during illumination were detected; The varies of the possible intermediates during degradation process were monitored with UV-VIS spectrum and HPLC. The possible mechanism of Congo red photocatalytic degradation is discussed combined with the data of MS.

本文选择有代表性的常见偶氮染料—刚果红作为研究对象，以自制TiO2为催化剂和小功率紫外灯为光源，在自制光反应器上确定了光催化降解的优化条件，并在这个优化条件下测定了降解过程中的脱色率、COD去除率和CO2生成量；利用数显酸度计、紫外可见分光光度计和高效液相色谱仪跟踪测定了降解过程中的可能产物，并结合质谱的数据初步探讨了刚果红的可能降解机制。

The agriculture soils of Baimahu ,Lushan and Zoujiao are the typical cases in the suburbs of Changde. Rice soil and vegetable plot are the two typical agriculture soils, we collected 6 samples according to the scientific method in these three areas. After air dry, contuse, screen and make soil samples, we tested their 9 indexs of PH, organic-substance, total phosphorus, quick-acting phosphorus. Then analysed the fertility condition of these areas. Then apply feasible suggestion of fertilizer after putting forward science and suiting measures to local conditions etc, by Compare with the documents and materials. Absorb to Model TAS-986 atom spectrophotometer examine with flame law (air - acetylene ) terms of potassium make deep discussion especially.

白马湖，芦山乡和陬郊的耕作土壤是常德市郊区耕作土壤的典型代表，在这三个地区分别就水稻土和菜地这两个基本的土地耕作类型按科学的方法共采集了6个样品，经过风干，研碎，过筛制成土样后，测定了它们的PH，交换性酸，有机质，全磷，速效磷等9指标，从而对该地区耕作土壤肥力状况进行分析，对比文献资料，总结分析结果，针对不同地区不同的土壤状况，提出了科学施肥，因地制宜等可行性建议，尤其是对TAS—986型原子吸收分光光度计用火焰法测钾的条件作了深入的探讨。

Major content of this work includes five aspects as follows:(1). Oxygen sensitive materials had been prepared by carrier covalence method, and the preparation mechanism of the materials had been investigated by FI-IR, SEM, and the detection experiment of oxygen. Then through the detection of oxygen, the sensitivity and stability had been estimated, and it was studied that the properties of materials would be affected by the various factors.(2). Oxygen sensitive materials had been prepared by carrier covalence-cross linking method, and the preparation mechanism of the materials had been investigated by FI-IR, SEM, and the detection experiment of oxygen. Then through the detection of oxygen and spectrophotometer, the sensitivity and stability had been estimated, and it was studied that the properties of materials would be affected by the various factors.(3). For the detection of gaseous oxygen, the fiber optical sensor on basis of oxygen sensitive materials had a respond time of 10s, detection limit of 5ppmand detection precision of 0.5%. The sensor had good repeatability and stability, a less delay and at least 1 year life-span.

本论文主要包括以下五个方面的内容：(1)载体共价法制备氧敏感材料：通过红外光谱、扫描电镜图谱和氧测试实验探讨该氧敏感材料的制备机理，通过氧测试实验评价该氧敏感材料的氧敏感性和稳定性，同时研究了各种因素对该氧敏感材料性能的影响(2)载体共价—交联法制备氧敏感材料：通过红外光谱、扫描电镜图谱和氧测试实验探讨该氧敏感材料的制备机理，通过氧测试实验和分光光度计评价该氧敏感材料的氧敏感性和稳定性，同时研究了各种因素对该氧敏感材料性能的影响(3)氧敏感材料在光纤气态氧传感器中的应用：该传感器的响应时间为10s，检测下限为5ppm，检测精度为0.5％，具有较好的重复性和稳定性，迟滞较小，使用寿命至少为1年，适合各种环境下气态氧浓度的检测。

From the results obtained in the study, the following conclusions can be made:The methylene blue and spectrophotometer method can capture the free radicals generated successfully. This is an effective way to measuring the free radicals quantitatively in cavitation, moreover it is easy to fulfill.The intensity of cavitation increases with reduction in the cavitation number. But the different mechanism of various enhancive processes (chemical and physical processes) results in different effect by cavitation number.The effect of operating parameters, such as inlet pressure, temperature and time on cavitation indicate that optimum operating conditions exist at the cavitation enhancive effect is maximum.It is important to optimize the structure of hydrodynamic cavitation reactor for achieving a desired effect. A reasonable geometric parameter can promote the enhancive effect for a fixed hydrodynamic condition.Ventilating appropriate gas to cavitating flow can increase cavitational intensity and hence promote the enhancive effect of hydrodynamic cavitation.

研究结果表明：亚甲基蓝分光光度计法，能够成功地捕捉到水力空化产生的羟自由基，是定量检测空化自由基简便易行的有效方法；空化程度随空化数的减小而增强，但对于不同的强化过程，因其强化机理不同，故空化数对强化效应的影响也不同；水力空化系统的压力、温度及时间等操作参数对空化强化效应的影响规律表明：在适宜的操作条件下可产生最佳的空化强化效果；空化发生器结构的优化设计是保证空化强化效应的重要前提，在一定的水力学条件下，合理的结构尺寸可以增强空化作用效果：往水流中适量加入干扰气体能够提高空化强度，增强空化强化效果。

In the biochemical analysis aspect, it is first time to use the simple fluorescence spectrophotometer to determine the protein in human serum by red alizarin as the probe of the RLS. The experiment shows that the system HSA-red alizarin has the maximum intensity of the RLS in Britton-Robison buffer of pH4.10 at maximum wavelength of about 360nm. The method has better linear range within 0.040-10.0ug/mL, determination limit is 0.040ug/mL. Moreover, We have selected three other dyes of chromotrope 2R, acid chrome dark blue and pyrocatechin violet as the probes of the RLS in determining protein in human serum and achieving satisfactory results.

在生化分析方面，作者首次使用简易960型荧光分光光度计，利用茜素红作为共振光散射光谱探针对人血清中的蛋白质含量进行了测定，测定结果显示在pH4.10的Britton-Robinson缓冲溶液，波长360nm处出现最大的共振光散射强度，其线性范围为0.040～10.0μg／mL，检出限为0.040μg／mL；另外，作者还筛选了其他三种可用作蛋白质测定的共振光散射光谱探针的有机染料：变色酸2R、酸性铬深蓝、邻苯二酚紫，并深入研究了测定的适宜条件和干扰情况，将这三种有机染料用于人血清中蛋白质含量的测定，均取得了满意结果。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素，结果表明，自组装胶束的形成机理受制备方式的控制，胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大，随着有机溶剂的水溶性的增强而减小；采用透射电镜观察胶束的形态结构，发现胶束呈具有核壳结构的均匀球形；采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度；通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能，发现与PELLA和PEDLLA的相比，PECL 降解速率较慢；随着共聚物中疏水嵌段含量的增大，纳米分散液的稳定性逐渐下降；PEG含量高的PEDLLA纳米分散液随着温度的升高，纳米分散液的粘度先下降然后增大，反之，没有规律性，且随着电解质Na 2SO 4浓度的增大，体系粘度增大。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。