光度计

Spectrophotometer was used for detecting the contents of total terpenoid components in the extracts of Alisma and various parts of elution with different kinds of macroporous resin, compared with acetylalisol B.

以乙酰泽泻醇B为对照品，用分光光度计测定泽泻提取物及不同树脂洗脱部位的总萜醇类成分含量。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料，分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA，并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

The expression of gene products hemoglobin in the cybrids were determined by means of benzidine staining method, immunofluorescent reaction and biochemical assay.

结果与电镜和流式细胞光度计的实验结果一致，表明珠蛋白基因产物血红蛋白住杂交细胞中，能够表达并成为融合细胞的标志。

The results show that the samples were X1 and type Y_2SiO_5 after calcined at 1300 and 1400℃,respectively.

通过X射线衍射、荧光分光光度计等分析测试仪器对样品的物相组成及发光性能进行了测试分析。

The influence on physiques of Camponotus japonicus which were kept indoors in winter was studied. Their survival rate was counted. The activity of SOD and POD was tested by spectrophoto-meter.

研究室内过冬对日本弓背蚁生活力的影响，统计法测定了其存活力的变化，分光光度计比色法测定了野生蚁及低温处理蚁超氧化物歧化酶、过氧化物酶等指标。

Compared with fresh samples, dried with silica gel, the genomic DNA was extracted from Castanea henryi leaves, which preserved for 10 days, 2 months, 4 months, 6 months, 8 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, by improved CTAB methods.

以野生锥栗幼叶为对照，采用硅胶干燥法，设置不同保存时间（10 d，2、4、6、8、10、11、12、13、14、15、16、17、18个月），对保存不同时间的锥栗叶样抽提基因组总DNA，利用紫外分光光度计及琼脂糖凝胶电泳检测提取DNA的浓度、纯度、得率及质量等指标，评价不同保存时间对基因组DNA的影响。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

The agreement for hue attribute between instrumental measurement and visual assessment is better than those for colorfulness and lightness.

并且在仪器测量和视觉评估的结果之间，色调的符合度优于彩度和亮度的一致性。标签色貌量度估计分光光度计光泽效应

Serum myocardial enzymes (CK, CK-MB, LDH) were measured with full automatic biochemical analyzer. The level of MDA, SOD and GSHPx were detected by spectrophotometer. Ultrastructure changes of myocardium were observed under electrion microscopy.

用全自动生化分析仪检测血清心肌酶CK， CK-MB和LDH含量；分光光度计检测线粒体MDA含量、SOD和GSHPx活性；透射电镜观察心肌超微结构的改变。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。