光度测定

A kinetic spectrophotometric method for the determination of trace oxalate was established, based on its inhibitory effect on the catalytic oxidation of indigo carmine with potassium bromate in the presence of V in 5 mmol/L H3PO4 media.

基于在5mmol/L磷酸介质中，草酸根对钒催化溴酸钾氧化靛蓝胭脂红褪色反应的抑制作用，建立了一种阻抑动力学光度法测定微量草酸根的新方法。

Each group was treated by intraperitoneal injection once every two days. The accumulation and metabolism of Al was analyzed by atomic absorption spectrum, expression of ED-1 by Kupffer cell was analyzed by immunohistochemistry.

采用原子吸收分光光度法测定铝元素在肝组织中含量的变化；免疫组织化学染色观察肝组织中枯否细胞的活化；RT-PCR检测凋亡相关基因Bax及Bcl-2 mRNA表达的改变。

9 healthy adult Holstein cows were selected as experimental cows and they were divided into 3 groups according to different milk lactations. Individual tracking method was used to observe and record the amount of Diptera insects. The blood was took on the tail vein under the aseptic condition and then the serum was extracted. The level of ACTH, COR, PRL, GH and NE in the serum were detected by the method of radioactive immunoassay method and fluorescence spectrophotometry.

方法］选择9头成年健康的荷斯坦奶牛作为试验牛，按不同泌乳阶段分3组，采用个体跟踪法观察并记录侵袭试验牛双翅目昆虫的数目，自尾静脉无菌采血，提取血清，采用放射性免疫法和荧光分光光度法测定血清中AGTH、COR、PRL、GH和NE的水平。

The left legs were taken for the biomechanics test on the EnduraTEC ELF3200, the biggest vertical deformation and stiffness were calculated, the number of empty osteocyte lacunas was calculated, and the cumulative absorbance value was compared between two groups.

分别于2，4，6，8周处死大鼠，取右侧股骨头标本，于EnduraTEC ELF3200生物力学材料动态力学性能测试系统行压力测定，计算样本纵向最大位移、刚度吸收等生物力学参数，计算空骨陷窝数，并行免疫组织化学法Bcl-2染色，比较不同组之间的累计吸光度值。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

In order to understand the lead and mercury content in Baby, sedative Chinese herb drugs, 179 preparations and 52 herbs of Chinese drugs were analyzed. Samples were prepared by Ash determination in lead and sulfuric acid-nitirc acid digestion in mercury, then determined by AAS.

将市售小儿惊风散类制剂179件及其药材52种，分别以灰化法及硫酸硝酸法处理后，利用原子吸光光度法(Atomic Absorption Spectrometry；AAS)测定铅及汞之含量。

To establish the method to determine lead in Polyaluminium chloride by flame atomic absorption spectrophotometry.

建立火焰原子吸收分光光度法测定聚氯化铝中铅的方法。

Based on a lot of documents, this thesis chooses several kinds of organic or drug small molecular, using resonance light-scattering technique, UV/Vis absorption spectra method, fluorescence spectra method and 1H NMR spectra method studied the interaction mechanisms of those small molecular and biopolymer. Base on the variation of the intensity of resonance light scattering is ratio to the concentration of the protein and DNA establishes several new determination methods of protein and DNA.

本文在查阅大量文献的基础上，选择了几种有机或抗癌药物小分子，用共振光散射光谱法并结合吸光光度法、荧光光谱法以及核磁共振光谱法研究了他们与蛋白质或核酸的相互作用，根据共振光散射光谱信号的改变值与蛋白质和核酸的浓度成正比的特点，建立了几种测定纳克级蛋白质或核酸的新方法。

This method was used to determine malachite green dye uptake of dying acrylic fiber. The results were accordance with the spectrophotometric method.

本方法用于测定MG上染腈纶的上染率，结果与分光光度法一致。

A method for determination of trace Phosphate in water by flow injection spectrophotometry with Molybdo phosphate and Malachite Green is described.

建立了流动注射-孔雀绿-磷钼杂多酸分光光度法测定水中痕量正磷酸盐的方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。