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光度测定

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METHODSUsing orthogonal design, total flavonoids in extract was as evaluation index, and ethanol concentration, extraction time, ratio of material to solvent and extration frequency were as inspecting factors to screened the optimum extraction technique, and total flavonoids content in extract was detected by spectrophotography.

方法]采用正交设计法以提取物中总黄酮含量为评价指标,以乙醇浓度、提取时间、固液比及提取次数为考察因素,筛选翻白草的最佳提取工艺。应用分光光度仪测定提取物中总黄酮的含量。

Analysis the changes of each groups mRNA expression of ICAM-1,VCAM-1 and eNOS .Naphthylamide Heavy nitrogen spectrophotography was adopted to detect thecontent of nitric oxide in the blood serum. To try to find out the mechanism of QDTMT prevent and cure function on AS.

采用蔡胺重氮分光光度法测定血清中NO含量,采用半定量RT一PCR的方法分别检测各组动物动脉壁匀浆及单个核细胞中ICAM一l、VCAM一1 mRNA的表达,以及动脉壁匀浆中eNOSmRNA表达,并分析造模及各药物组上述指标mRNA表达的变化;进一步探讨QDTMT防治As作用的机制。

Second, the light adsorption values of bursin with different concentrations at different wavelengths were observed by using ultraviolet spectropolarimeter.

另一种是采用紫外分光光度法测定不同波长下不同浓度囊素的吸光值,建立波长23Onm时囊素浓度与光吸收值之间的回归方程。

Methods: The culture mediums in 1% SBOS, 0.36% stachyose, 0.52% sucrose and 0.11% raffinose were prepared respectively, then Bifidobacteria and Enterobacillus were inocuted into the mediums.

按1%大豆低聚糖、0.36%水苏糖、0.52%蔗糖、0.11%棉子糖含量配制培养液,分别接种各试验菌株,在0、12、24小时测定各培养液吸光度A值(分光光度计,550nm)和pH值。

Gas chromatography with sample-pretreatment by solid-phase micro-extraction and with pulse flame photometric detection was applied to the determination of 6 stenchy sulfides(hydrogen sulfide,carbon disulfide,methanethiol,1,2-ethanedithiol,dimethylthioether and carbonyl sulfide) in polluted air.

应用固相微萃取预处理技术,采用脉冲火焰光度检测器-气相色谱法测定污染空气中硫化氢、二硫化碳、甲硫醇、1,2-乙二硫醇、甲硫醚、羰基硫6种恶臭硫化物。

To judge the existance of sodium formaldehyde sulfoxylate in food by comprehensive checking of HCHO content and SO_2/HCHO content ratio is accurate and scientific.

采用直接蒸馏滴定———AHMT分光光度法联合测定食品中的吊白块含量。

Derivative Spectrophotometry ; Simultaneous determination ; Cu Zn ; Meso tetra (4 phenyl sulphonate) Porphyrin ; Foodstuff

导数光度法;同时测定;铜锌; meso一四(4—磺基苯基)卟琳;食品

We studied some new colour reactions for the first time. Amino-Gacid chlorophosphonazo, sulphonic acid amide chlorophosphonazo and phippuric acid chlorophosphonazo were used to establish flow injection spectrophotometric methods of Pd in ores.

我们首次探索了一些前人未研究过的新显色体系,使用氨基G酸偶氮氯膦,磺胺偶氮氯膦,对马尿酸偶氮氯膦建立了测定矿石中Pd的流动注射分光光度法。

At the same time the general activity of SOD and the content of MDA and TChE in mice brain's tissue was measured with UV.

同时,利用紫外分光光度法,测定脑组织内总超氧化物歧化酶的活力、丙二醛的含量、乙酰胆碱酯酶的含量。

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化,发现药物在一定浓度作用下,DNA指数无明显变化;丹参使C2-M期细胞分布增多,G2-M期延长;川芎嗪使G2-M期细胞分布增多,G2-M期、S期延长;药物使细胞群体倍增时间延长,与浓度呈明显直线正相关。

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