光度测定

objective:to make a comparative analysis of the total saponins content in polygalaceae phloem and xylem parts to guide clinical treatment.methods:we determined the polygalaceae total saponins content at the 575 nm with oleanolic acid as a reference substance and vanillin-perchoric acid as a developer by using uv-visible spectrophotometry.results:the total saponins content of xylem parts was 3.33%,and that of polygalaceae phloem was 0.69%.conclusion:the total saponins content in polygalaceae xylem parts is much more than that of phloem parts,the total saponins in polygalaceae mainly exists in xylem parts.

目的：对远志原药材木部及皮部中总皂苷含量进行比较分析，用以指导临床用药。方法：以齐墩果酸为对照品，香草醛－高氯酸为显色剂，采用紫外－可见分光光度法在575 nm处测定远志总皂苷含量。结果：以此方法对远志木部及皮部中总皂苷含量进行比较、分析，为远志皮部总皂苷含量为3.33 %、木部总皂苷含量为0.69 %。结论：远志中皮部总皂苷含量远远大于木部，远志总皂苷主要存在于根皮中。

An UV spectrophotometry for determining total coumarins in Angelica polymorpha Maxim.

建立紫外分光光度法测定白根独活中总香豆素的含量。

Objective: To separate and determine the content of protocatechuic aldehyde in Danshen Injection by TLC-UV method.

目的：建立薄层层析-紫外分光光度法测定丹参注射液中原儿茶醛的含量。

An UV-spectrophotometry determination method for pyrogallic acid content was studied.

研究了用紫外分光光度法测定焦性没食子酸含量的一种新方法。

The extraction process and content examination were studied with the methods of orthogonal test and spectrophotometry compared with Rutin; then the total flavonoids from Ramulus Mori were extracted with the methods of phytochemistry and examined.

以芦丁为对照品，运用正交设计试验及分光光度法，对桑枝总黄酮(the total flavonoids from Ramulus Mori，RMTF)的提取工艺、含量测定进行了探索；然后，采用植物化学方法提取并定性检测桑枝总黄酮，进而开展动物降血糖药理试验。

Trace Ru could sensitively catalyze the fading reaction of chlorosulphophenol azo rhodanine with KClO3 in H2SO4 medium in water bath at 85 ℃, and the fading level was in direct proportion to Ru in certain content range. Based on this, a novel determination method of trace ruthenium was established by catalytic kinetic spectrophotometry.

H2SO4介质中，水浴85℃条件下，痕量Ru能灵敏地催化KClO3氧化氯磺酚偶氮罗丹宁的褪色反应，且褪色程度与钌含量在一定范围内成正比，据此建立了催化动力学光度法测定痕量钌的新方法。

Methods:We determined total flavonoids contents in leaves of Acer Saccharum by ultraviolet and visible spectrophotometry.

采用紫外-可见分光光度法对糖槭叶中总黄酮含量进行测定。

Separation of the sapindus-saponin from natural sapindus pericarps is very difficult, a rapid and simple determination method for total-sapindus-saponin is not founded.

为解决无患子皂苷分离较困难，无快速简单的检测方法等问题，本研究采用超滤膜分离和泡沫分离等新型分离技术对无患子皂苷进行分离纯化，并采用分光光度法测定无患子总皂苷。

Based on the shortcomings of the COD determination method revised in National Standard, some improvement measures of the standard method, including improvement of slaking phrases, research of substitution catalysts and elimination of chlorine ion disturbance, are compared and discussed. Afterwards, some common substitution COD determination methods, such as correlation coefficient analysis method, electrochemistry analysis method, spectrophotometric analysis method and flow inject analysis method, are introduced.

在论述了国标中COD标准方法不足之处的基础上，本文就标准方法的众多改进措施分类进行了比较与探讨，包括消解方法的改进、替代催化剂的研究以及氯离子干扰的消除，然后介绍了相关系数法、电化学法、分光光度法和流动注射法等COD测定替代技术。

Based on this,first part of this work has established spectrophotometer and laser induced fluorescence spectrographic methods based on the liquid-core waveguide,and made it successfully apply to detecting iron and the cupric ion concentration.

基于此，本工作第一部分建立了基于液芯光纤的分光光度法和激光诱导荧光光谱法，并成功地应用于1，10邻二氮菲对铁的检测和催化荧光动力学方法对铜离子浓度的测定。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。