光度测定

METHODS:Aminopyrine and antipyrine in Antongding injection were determined by radial basis fuction neural network UV spectrophotometry,barbitial was determined with absorbance substraction technique.

采用径向基函数紫外分光光度法同时测定安痛定注射液中的氨基比林和安替比林，并结合吸光度减技术测定巴比妥。

Third, sodium sulfoxylate formaldehyde is a kind of bleaching powder used in industry, which is prohibited from adding to food. In this part, we take it as an object to study, by using ion chromatography instrument which is equipped with autech DS-PLUS?

本章利用荧光光度法和紫外吸收分光光度法研究了核酸对环丙沙星-Tb~(3+)体系的荧光增强效应及其影响因素，探讨了其作用机理，提出了环丙沙星-Tb~(3+)配合物与核酸的作用模型，并建立了DNA的选择性测定方法，并将其运用于合成样与血清中DNA含量的测定。

A sensitive spectrophotometric method for the determination of trace amounts of chlorine dioxide based on the fading reaction of chlorophenol red by chlorine dioxide has been developed in the alkaline solution.

在弱碱性条件下，二氧化氯(ClO2 )氧化氯酚红使其褪色，于 5 75nm波长处测定剩余氯酚红的吸光度，当用试剂空白参比时，吸光度的降低值与ClO2 的浓度呈直线关系，其它氧化剂在测定条件下无干扰，方法有较高的灵敏度和很好的选择性。

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

It is that:(1) the related compounds in Metronidazole Buccal Tablets prepared by Chongqing Medical College can be tested by determining (321nm) the total peak area of the impurities;(2) The related compounds in Metronidazole Disodium Phosphate for Injection prepared by Chongqing Medical College can be test by determining (254nm) the total peak area of the impurities and the peak area of the largest impurity;(3) Ceftriaxone polymer in Ceftriaxone Sodium for Injection prepared by Chongqing Keru Pharmacy Co.

结果如下：①用HPLC一UV法，在321nm处检测，用归一化法计算，控制各杂质峰面积的和，可检查重庆医科大学的注射用甲硝吟磷酸二钠中的有关物质：②用HPLC一UV法，在254nm处检测，控制杂质峰面积的和及控制最大杂质峰面积，可对重庆科瑞制药股份有限公司的注射用头饱曲松钠中的有关物质实施控制；③用HPLC一Uv法，在254nm处检测，按外标法计算，可检查注射用头抱曲松钠中的聚合物；④用紫外分光光度法，在277nm波长处测定吸收度，按C6H9N303的吸收系数为377计算每片标示百分含量，可测定重庆医科大学的甲硝哇口颊片的含量均匀度；⑤用紫外分光光度法，在277run波长处分别测定释放溶液20min、50min和90min的吸收度，按甲硝哇(C6H剥303)的吸收系数为377计算释放度，可检查重庆医科大学的甲硝哇口颊片的释放度。

It is very significant and positive to search rapid methods of determination of nucleic acid.

核酸的定量测定是研究核酸的基础，目前核酸的测定方法有吸光光度法[2]、紫外光度法[3]、荧光分析法[4]和化学发光法[5]等。

In order to provide some scientific data for judging biological toxicity and bioavailability caused by heavy metals in sediments in Guilin, China, for the first time, the authors sampled sediments in different water areas in Guilin, and determined AVS by P-Amino dimethyl aniline dihydro chloride photometry through a set of acidification-ventilation-absorption AVS reaction unit, determined copper, lead, zinc, cadmium and nickel with tomic absorption spectrophotometry, mercury and arsenium with atomic fluorescence, and cipangopaludina cathayensis was selected as the tested species for bioavailability experiment.

首次以桂林水系沉积物为研究对象，通过酸化－吹气－吸收装置，用对氨基二甲基苯胺光度法测定沉积物中AVS含量，用原子吸收分光光度法测定SEM和间隙水中Cu、Pb、Zn、Cd、Ni含量，用原子荧光法测定Hg、As含量。

The contents of Mn, Cu, Fe, Zn in the leave, flower, seed of Impatiens balsamina induced by space flight are determined by flame atomic absorption spectrometry. The measure parameters for FAAS are optimized.

采用火焰原子吸收分光光度法测定了航天诱变凤仙花的茎、叶、花、种子中Mn、Cu、Fe、Zn四种微量元素的含量，优化了原子吸收分光光度法测定参数。

In this paper,the authors compared extraction spectrophotometric method with gravimetric method in the determination of naphthenic acid in oil refining sewage,and found that the former method is better than the latter both in precision and operation.

讨论了萃取分光光度法测定炼油污水中环烷酸含量的测定条件和干扰因素并与重量法进行了比较，萃取分光光度法的精确度比重量法高而操作比重量法简单。

Methods Conjugated with protein precipitation with trichloroacetic acid, BCA method was used.

使用三氯乙酸沉淀蛋白质后，运用BCA法，在570 nm波长处，分别测定标准蛋白质应用液与样品稀释液的吸光度值，基于测定液中蛋白质含量与其吸光度值呈正比关系，计算出样品中蛋白质的含量。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了