光度

Potassium Guaiacolsulfonate ; syrupus phenergani tussis ; first derivative spectrophotometry

愈创木酚磺酸钾；非那根止咳糖浆；一阶导数分光光度法

Methods:tlc was used by the same rf of the color for the identification,the content of uncaria alkaloid in rhynchophylline was determined by ultraviolet spectrophotometry.

采用tlc通过显色剂的显色比较有相同的rf进行鉴定；采用紫外分光光度法对钩藤总碱中的钩藤碱进行含量测定。

The content of uncaria alkaloid in rhynchophylline was 0.71% measured by ultraviolet spectrophotometry at 246.0 nm wavelength.

结果：本品定性鉴定薄层色谱特征明显，与标准品有相同的Rf；紫外分光光度法在246.0 nm波长处测得钩藤总碱中钩藤碱的含量为0.71%。

Results:tlc characteristic of qualtative identification of this goods was apparent,with the same rf to standard samples.the content of uncaria alkaloid in rhynchophylline was 0.71% measured by ultraviolet spectrophotometry at 246.0 nm wavelength.conclusion:this method was found to be rapid,simple,economic,accurate and reproducible.

结果：本品定性鉴定薄层色谱特征明显，与标准品有相同的rf；紫外分光光度法在246.0 nm波长处测得钩藤总碱中钩藤碱的含量为0.71%。结论：此方法快速、简便、经济、准确、重现性好。

The results showed that the influence of time and temperature to absorbency is visible about standard solution of chlorogenic acid, but solution of the tobacco sample is unconspicuous.

研究了显色时间和显色温度对多酚总量检测的影响，实验证明，显色温度和显色时间对绿原酸标准溶液的吸光度影响较大，而对烟草样品溶液影响较小。

The therapeutic effect of HYJDT on glomerulosclerosis was observed by way of five aspects:(1) Effect on renal tissue morphology;(2) Effect on urine protein and blood creatinine and blood uria nitrogen ;(3) Effect on superoxide dismutase and malonydialdehyde in reual cortex;(4) Effect on matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-l (TIMP-1) in glomerulus;(5) Effect on MMP-9 mRNA and TIMP-lmRNA and type-1 plasminogen activator inhibitor (PAI-1) mRAN in glomerulus.

用药治疗12周取肾，HE、PAS染色，在光镜下观察肾组织形态学变化，并在透射电镜下观察肾小球超微结构的变化。用半自动生化分析仪检测肾皮质匀浆中SOD活力、MDA含量。用免疫组化技术检测肾小球中MMP-9、TIMP-1的蛋白表达，用原位杂交技术检测MMP-9mRNA、TIMP-lmRNA、PAI-lmRNA的基因表达。免疫组化和原位杂交结果，采用计算机图象分析系统测量各自的平均吸光度。所有的实验数据均用SAS6.12统计软件包进行统计学处理。

Stems and leaves were determined and compared by al(no3)3 colorimetry and hplc. the method with ultrasonic wave was applied to extract flavonoids from the leaves and stems of urtica dioical l.. with the contents of flavonoids as the index, the best extraction conditions were selected out. results the best extraction conditions were as follows:soaking the raw material with ethanol(80%, v/v)-water solution (1∶30) at 60℃.

方法分别采用al（no3）3络合分光光度法和hplc法测定异株荨麻茎叶中黄酮类化合物的含量，并对测定结果进行比较；同时以黄酮类化合物的含量为指标，优选出了超声波辅助法提取异株荨麻茎叶中黄酮类化合物的最佳条件。

The culture filtrate of strain LN-176 was tested for light absorption at 260nm and 280nm, and the results showed that the protein content in the culture filtrate significantly increased. After ammonium sulphate precipitation, the supernatant and the precipitation were separated to testify their antagonistic activity, and it was found that the precipitation was inhibitory to Ustilago hordeum , but the supernatant not.

LN-176菌株的发酵液在260nm和280nm下进行吸光度测定，结果表明发酵液中蛋白质含量明显升高；发酵液经硫酸铵盐析后，取上清液和沉淀物分别做抗菌试验，发现沉淀物对青稞散黑穗病菌有拮抗活性而上清液无活性。

The axial interaction of vanadyl meso-tetraphenylporphyrin and nickel meso-tetraphenylporphyrin with different Lewis basic solvents was investigated by using an ultraviolet-visible spectrophotometric method.

应用紫外可见分光光度法研究了氧钒对四苯基卟啉和镍对四苯基卟啉与Lewis碱性溶剂的轴向配位作用。

To control the slope of standard curve in spectrophotometry by using the two kinds of graphics of Youden,we can directly find out the quality of the data,accuracy and precision of the measuring point.

应用尤登双样图对分光光度法进行标准曲线斜率的控制，能简单直观地了解数据质量，看出测点的准确度和精密度，可用同心圆确定测点所代表的标准曲线的斜率是否可接受

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。