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The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb,用Ang Ⅱ诱导促进其增殖,采用MTT法检测细胞增殖;羟脯氨酸试剂盒检测胶原含量;ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量;流式细胞仪检测细胞周期;硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

Total nitrogen and total phosphorus in water were determined by microwave digestion and flow injection spectrophotometry using basic potassium persulphate as digestion solution.

以碱性过硫酸钾溶液为消解液,采用微波消解-流动注射分光光度法测定水中总氮和总磷。

The molecular structure was clarified by IR, which revealed the existence of ether bonds. The content of tartaric acid and unreacted maleic acid were detected by spectrophotometer. The effect of some factors on the yield of ESA and scale inhibition efficiency of PESA was investigated.

利用红外光谱表征了该产物结构,表明分子链中含有醚键;利用紫外-可见分光光度法测得体系内副产物酒石酸和未反应的马来酸的含量,并系统地研究了各因素对中间产物环氧琥珀酸钠的产率和最终产物聚环氧琥珀酸钠阻垢性能的影响。

Mm, 5 μm and a UV detector. Elution was performed at a flow rate of 1 mL/min, with a gradient of methanol-acetonitrile-water starting at 80∶10∶10, increasing linearly to 86∶10∶4 in 35 min, then returning to the initial conditions in 5 min. Elution of phenacyl esters was monitored by absorbance at 254 nm. Pentadecanoic acid was used to be an internal standard.

分析所用色谱柱为ZORBAXSB C18柱,以十七酸作为内标,甲醇-乙腈-水为流动相,采用梯度洗脱(梯度洗脱程序为甲醇-乙腈-水由80∶10∶10在35 min内线性变化到86∶10∶4,然后在5 min内恢复到起始比例,流动相流速为1 mL/min),通过测定苯乙酰甲酯在254 nm处的吸光度值来测定脂肪酸含量。

The hydroxyl free radical scavenging rate was determined by phenanthroline spectrophotometry, and the antilipoperoxidant was determined by colorimetry.

采用邻二氮菲分光光度法测定经自由基的清除率,比色法测定其对脂质过氧化的抑制作用。

The iron content was determined by phenanthroline photometry, and T(2) values as well as relaxivity evaluated with a clinical MR system at 1.5T.

用菲咯啉光度法测定铁含量,并用临床用核磁共振系统在1.5T强度下测定T(2)值和弛豫效率。

Condition filter experiments for determination of the iron by phenanthroline spectrophotometry in the genral textbook are single factor experiments, and there are some problems in the experimental design.

普通教材中邻二氮菲分光光度法测定铁条件选择实验都是单因素条件实验,不够全面,实验设计中也存在一些问通。

A catalytic kinetic spectrophotometric method has been established for the determination of trace Mn, based on the catalytic action of Mnon the oxidation - reduction reaction between KIO4 and KI in the presence of 1, 10 -phenanthroline and in HAc-NaAc buffer solution.

本文研究了HAc-NaAc缓冲溶液中,在1,10—菲啰啉激活作用下,锰对KIO4-KI氧化还原反应的催化动力学条件,建立了催化动力学光度法测定痕量锰的方法。

In the rapid ampoule determination of low concentration iron in water phenanthroline is used as developing agent.

测定条件;油田水分析;中低浓度;吸光度;准确度;铁含量;快速测定法;铁水样;目视比色法;灵敏度

Additionally, Pb-pheophytin was non-fluorescent and could result in the fluorescence intensity quenching of pheophytin.

通过光度动力学测定,分别求得铅与脱镁叶绿素a、脱镁叶绿素b和13〓-羟基脱镁叶绿素a的反应速率常数为2.0、0.78和1.86mol〓 ls〓。

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