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Methods The percentages of apoptotic bodies and the DNA lytic rates in mouse thymocytes and splenocytes were detected with flow cytometry and fluorospectrophotometry, respectively.

采用流式细胞术和荧光分光光度法分别检测小鼠胸腺和脾淋巴细胞凋亡小体百分率和DNA裂解率。

However 528 nm and (dR716-R496)/(R716+R496) were the most important waveband and index to evaluate the variation of Fv/Fm on Mangifera indica and Decussocarpus nagi. At water deficit photosynthetic capacity and stomatal conductance reduced by decreasing soil water potential.

在平地结果显示,不同生态习性之植物其Fv/Fm对温度及光度之反应各异,并得知528 nm为推估芒果Fv/Fm萤光变化的重要波长,其反射率一次微分值为推估芒果Fv/Fm之最佳指数,NDVIdR716-496则为推估竹柏Fv/Fm最准确之指数。

In order to mensurate the certain amount of iron,I apply the spectrum-kurtosis technic on the basis of using orthophenanthroline spectroscopy,this method can avoid to be disturbed by oher ions and raise the accuracy.

在邻二氮菲光度法的基础上,引入光谱峰面积技术,对微量铁进行定量测定,该方法避免了有关离子的干扰,提高了准确度。对于面粉、奶粉和肝等食品样品进行分析,结果令人满意

With the experiments done in this research, effects on visual performance at mesopic region were described, which belongs to the necessary basic research work for developing the new mesopic system.

本论文研究通过选用不同的照明水平、目标大小和目标偏心度的条件进行实验,其研究成果能实现对影响中间视觉状态下视觉功能的一些因素的描述,这是建立中间视觉光度学所必须解决的基础研究工作。

The creative point of this dissertation consists firstly in that monochromatic stimuli instead of combined spectral power distribution were used as visual object, and spectral luminous efficacy curves were drawn from the performance-based method; Comparison between performance-based method and brightness-matching method was then done and therefore connection between these two methods was developed; One more creative point is that the analysis based on the theory of neurophysiology and psychophysics leads to the conclusion that contribution of the colour channel during visual information processing is reflected from performance-based method, which should be considered when CIE develops the new mesopic photometry system.

论文的新颖之处在于实验中使用了单色光谱代替复合谱或物体色作为视觉目标,在分析中间视觉状态下视觉功能的光谱响应特性的基础上反推了人眼的光谱光视效率函数;论文更进一步验证了基于视觉功能法和传统的视亮度匹配法之间的联系,在两种方法之间构造了一座桥梁,为基于视觉功能法的使用初步建立了理论依据;同时论文创新地采用心理物理学的方法进行实验和结合物理学和视神经生理学的知识对实验结果进行分析,从而从视觉信息处理的神经机制层面证明了基于视觉功能法获得的结果包含了视觉颜色通道效应,并对CIE在建立新的中间视觉光度学系统时要充分考虑这一视觉颜色通道的影响提出了建议。

By dehydrochlorination reaction the poly[(2-methoxyl-5-octyloxy)phenylene vinylene] is synthesized and the material"s structure is characterized by nuclear magnetic resonance spectroscopy, infrared absorption spectrum, ultraviolet and visible spectrophotometry. At the meantime, the fluorescence spectrum is measured and the mechanism of fluorescence occurring is analyzed. Thermal Gravimetric Analyzer"s results show that the MO-PPV"s thermal stability is fine. Also the experiment of resolvable property indicates that the MO-PPV can well dissolve in chloroform. Through analyzing the film"s surface by Metaloscope and Atomic Force Microscope, the good filming is found.

通过脱氯缩合法合成了聚(2-甲氧基-5-辛氧基)对苯乙炔,并分别用核磁共振波谱法、红外吸收光谱法、紫外-可见分光光度法等对其进行了结构表征;测量了样品的荧光光谱,分析了荧光产生的机理;热失重仪分析了材料的热稳定性,研究表明MO-PPV材料具有良好的热稳定性;溶解性实验发现材料在氯仿中溶解性较好;金相显微镜和原子力显微镜研究了薄膜的表面形貌,发现该材料的成膜性良好;材料的导电性研究表明,固体材料在掺杂下才可以表现出导电特性,而配成溶液的材料导电性因溶剂的不同而不同,并随溶解时间的长短发生变化。

Rats 20 days after birth the TGF-βexpression in the metaphysis:positive signals in cells,dark blue,located in cytoplasm;positive expression intensity of the use of quantitative analysis of optical density.

仔鼠生后20天时TGF-β在干骺端的表达:阳性信号的细胞呈深蓝色,定位于细胞浆;阳性表达强度采用平均光度进行定量分析。

In this paper, the determination of trace elements including copper, manganese,cobalt, nikel and chromium in lead metasilicate by atomic absorption spectroscopyhas been investigated.

本文提出用火焰原子吸收分光光度法测定硅酸铅中的微量元素铜、锰、钴、镍和铬,并探索了样品中可能存在的干扰元素及溶剂介质酸度对测定的影响。

Atomic absorption spectrophotometry ; determination ; microelement/lead metasilicate

原子吸收分光光度法;测定;微量元素/硅酸铅

MTS/PMS microcolorimetry was applied to assess A490 absorption factor of HASMCs cell. In all groups, cell cycle distribution was detected by flowcytometry.

MTS/PMS微量比色法检测各组HASMCs细胞A490吸光度,流式细胞仪检测细胞周期分布。

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