光度

A new method was developed for detection of the hydroxyl radical in Fenton reaction by bromocresol green oxidative assay. After oxidized by hydroxyl radical, the absorbance of bromocresol green is decreased apparently.

利用Fenton反应产生的羟基自由基与染料试剂溴甲酚绿(Bromocresol green， BG)发生氧化反应后使其吸光度值降低，建立了一种可间接测定羟基自由基的新方法。

Guangdong college of pharmacy,guangzhou,guangdong 510006,china;henan university of traditional chinese medicine,zhengzhou,henan 450008,chinaabstract:objective to establish a method for the determination of the content of total alkaloid in nao mai tong recipe.methods acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.results the calibration curve of the absorbance was linear with the concentration of ligustrazine in the range of 0.031～0.153 mg/ml(r=0.9998).the average recovery of ligustrazine was 100.95％,rsd=1.75%.

作者单位：广东药学院中药化学与分析教研室，广东广州 510006；河南中医学院，河南郑州 450008 目的建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法，以溴甲酚绿缓冲液作为生物碱的显色剂，测定样品溶液在417 nm波长处的吸收度。结果对照品川芎嗪质量浓度在0.031～0.153 mg/ml的范围内与吸光度呈良好的线性关系，r=0.9998。平均回收率为100.95％，rsd为1.75％。精密度和稳定性良好。

Keywords Catalytic spectrophotometry, surfactant, bromocresol green, silver

本法是迄今为止催化光度法测定银的最灵敏方法之一。

UV-Vis and continuous variation plot method were used to study the complexation of γ-cyclodextrin with bromocresol green, and the stoichiometric ratio of the complexation was to be 1∶2. Thermodynamic analysis results show an inverse relationship between temperature and complexation constant, and the change of enthalpy, entropy and free energy of the complexation were -39.988 kJ/mol, 86.400 J/ and -14.245 kJ/mol, respectively, which indicating that hydrophobic effect was the main force to form the complexes.γ-Cyclodextrin and bromocresol green complexes were examined by nuclear magnetic resonance, infrared spectrum and molecular modeling analysis, and was may be the group included in γ-cyclodextrin.

采用紫外-可见分光光度法和等摩尔连续变化法研究了γ-环糊精与溴甲酚绿的包合作用，确定了包合物形成的化学计量比为1∶2；采用热力学方法分析了温度与包合常数之间的关系，计算了包合过程的焓变、熵变及自由能变化分别为-39.988 kJ/mol， 86.400 J/和-14.245 kJ/mol，这表明疏水作用力为主要驱动力；采用核磁共振、分子模拟和红外光谱法对包合物进行了研究，确定了包合物的形成，分析认为这可能是基团进入γ-环糊精腔内导致增色效应。

Based on the associated reactions of bromothymol blue with cationic surface- active agent when put in phosphate buffer solution of pH7.5～8.5 and fades, a new way for determination of cationic surfactant by Spectrophotometric methods was established.

摘 要：基于溴百里酚蓝在 pH7.5～ 8 5的磷酸盐缓冲介质中，与阳离子表面活性剂发生离子缔合反应，而使溴百里酚蓝褪色这一现象，建立了分光光度测定阳离子表面活性剂的新方法。

The conclution is confiemed mutually by the tested result of TBAB distribution coefficients in the differen phases in Benzene/TBAB/water/KBr system,the distribution coefficients is tested by the method of Bromothymol Blue Spectrophotometric Determination.

得到结论同溴百里酚蓝分光光度法测定苯/TBAB/水/KBr体系各相态中TBAB分配系数的结果相互验证。

Methods: Through the reaction between protein and bromthymol blue, the absorption of the system was measured at 615 nm.

利用溴百里酚蓝与蛋白质的结合反应，在615nm波长处测定体系的吸光度。

The determination of MD1 concentration in aqueous solution by using bromthymol blue indicator method is described and a linear relationship is observed between absorbance and solution concentration ranged in 10-300 mg/L.

介绍了溴百里酚蓝为指示剂测定水溶液中MD 1质量浓度的方法，在10～300mg/L范围内溶液吸光度与浓度之间有良好的线性关系。

Bromthymol blue ; spectrophotometry ; protein

溴百里酚蓝；分光光度法；蛋白质

With haematoxylin as the standard, the determining method of content of total hydroxybenzene in Caesalpinia Sappan L. was established by spectrophotometry.

首先以苏木精为标准物，建立了分光光度法测定总酚含量的分析方法，回归方程为C=10.2569A-0.07901，相关系数为0.9999。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。