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Methods Cassia Seed was soaked in chromium trichloride aqueous solution of different concentration (from 0 to 3600 mg/L) at 37℃ for 12 hours.

方法用不同浓度的三氯化铬水溶液,37℃浸泡决明子过夜,收获浸泡后的决明子,作为0h时间点的样品,然后分别收获24、48、72、96h的决明子芽,用石墨炉原子吸收分光光度法测定铬含量。

The results indicate seawater concentration has no effect on absorbency, pH, relative density, dry matter and water contents in Aloe vera leaves. While, seawater stress causes increased MDA and membrane penetration, decreased phytochroms, prolines and NO_3~--N. It is beneficial to plant growth when irrigation was done with 10% seawater, because of higher ratio of K~+/Na~+ and lower ratio of Na~+/Ca~(2+). In addition, the polysaccharide contents in Aloe vera leaves increase twice and triplication under 25% and 50% seawater irrigation respectively, the barbaloin contents increase over triplication in var. 75% seawater and over twice in var. 50% seawater and var. 100% seawater. However, when seawater concentration exceeds 25% the growth of plant was inhibited, biomass decreased, flowering time was deferred, Ca~(2+) content decreased caused by Na~+ accumulation in leaves.

结果表明:在海水胁迫下,库拉索芦荟全叶汁的吸光度、酸度、相对密度、总固形物和水分含量总体上受海水浓度的影响不大;但芦荟叶的丙二醛含量和质膜透性增加,叶绿体色素、脯氨酸和硝态氮含量减少;在10%海水浇灌下,芦荟叶细胞具有较高的K~+/Na~+比和较低的Na~+/Ca~(2+)比,对库拉索芦荟的生长有一定的促进作用;在25%海水和50%海水浇灌下,芦荟多糖含量提高了2-3倍;在75%海水浇灌下芦荟甙含量增加了3倍多,在50%海水和100%海水灌溉下,其含量也增加了2倍多;当海水浇灌浓度超过25%后,库拉索芦荟的生长明显受到抑制,生物量降低,抽苔期推迟,同时,海水浓度的增加也导致芦荟叶中累积大量Na~+,从而使得芦荟叶中Ca~(2+)含量降低,但Mg~(2+)并没有受到海水浓度的影响;用浓度低于75%的海水浇灌库拉索芦荟可以降低剖面深度在0-100cm之间的土壤盐度;其抗氧化保护酶中超氧化歧化酶的酶活力相对保持稳定,过氧化物酶的酶活力在10%海水浇灌下明显提高,随后则下降,而过氧化氢酶的酶活力则随海水浓度的升高而下降。

The magnitude of the decrease of absorbance at 410 nm is a measure of trypsin inhibitor activity.

基础于此原理建立了测定胰蛋白酶抑制剂活性的紫外分光光度法。

Content of trypsin inhibitors in extracts of cyanobacterium from Taihu lake was determined by UV spectrophotometry in this paper.

采用紫外分光光度法对太湖蓝藻提取液中的胰蛋白酶抑制剂进行了含量测定,测定的线性范围在 4~ 4 0 μg/mL 。

However, when reaction time is fixed, this system can follow Lambert-Beer Law within visible spectrum. Furthermore, it can not be affected by temperature. Based on these findings, CTAB Turbidness Method, a quick method to determine hyaluronic acid contents in fermentation broth is established.

研究表明,随着反应时间延长,反应体系的吸光度逐渐升高,但当反应时间固定后,在可见光区反应体系能较好的遵循朗伯-彼尔定律,并且不受温度的影响。

RESULTS:The 50%ethanolic extracts of 20 Chinese crude drugs were examined for inhibitory activity on tyrosinase.

应用多巴色素法测定对酪氨酸酶的抑制活性,底物为L-酪氨酸,在490 nm处测定吸光度

Quantitative analysis of protein adsorption: The PS suspension containing lysozyme was subjected to ultracentrifuge (15 000 r/min) to collect clear aqueous solution. The lysozyme concentration in the clear solution was measured by spectrophotometry at 280 nm. The amount of protein adsorbed on PS surface was calculated based on the decrease in the protein concentration in the supernatant solution.

蛋白吸附定量分析:采用高速离心(15 000 r/min)法从含溶菌酶蛋白的聚苯乙烯悬浮液中分离出上清液,用紫外(280 nm)分光光度法测定经聚苯乙烯微球表面吸附后,液相蛋白质量浓度的下降值,由此推算蛋白在聚苯乙烯表面的吸附量。

Acid brown NR could combine with albumin such as bovine serum albumin or human serum albumin in acidic Britton-Robinson solution and produce a undissolved precipitation.

采用分光光度法研究了酸性棕NR 与血清白蛋白的结合反应。

Heated in water bath,uranyl ion and nitrate radical adsorbed on resin are replaced into solution by sodium bisulfate,then are determined by titanous method and spectrophotometry,respectively.

在水浴加热条件下,用硫酸氢根将吸附在树脂上的铀酰离子和硝酸根离子置换到液相中,之后分别采用亚钛法、分光光度法进行测定。

The optimum condition of spectrofluorimetric determination of uranyl ion in pyridine 2,6 dicarboxylic acid β cydodextrin system is studied.

研究了吡啶 2 ,6 二羧酸β环状糊精体系荧光分光光度法测定铀酰离子的最佳条件以及某些稀土共存离子对荧光体系的干扰,并应用于人工样品的测定。

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