光密度

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

It is important to analyzing experimentation's results The results of blood model test indicated that this system can measure the changes of blood volume and oxygenation with a high responsivity.

对该系统进行血液模型实验，实验结果表明该系统能够正确反应血液中血氧和血容的变化，并且具有较高的灵敏度(当实际血容变化约7%，测量到的以光密度表示的血容变化为0.1)。

The rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule（4.0、2.0、1.0g/kg）, then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the OD numerical value was detected.

(1)抗炎实验：将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只，补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg)，对照组给予蒸馏水，采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高，测定光密度值；(2)镇痛实验：40只小鼠分组及给药同抗炎实验，采用腹腔注射醋酸致小鼠出现扭体反应，纪录扭体次数并计算镇痛百分率；(3)抗前列腺炎实验：将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组，各10只。

Methods ①anti-inflammatory trial: 40 rats were randomly divided into control group, high-dose bushenlizhuo capsule group, middle-dose bushenlizhuo capsule group and low-dose bushenlizhuo capsule group(10 rats in each group).the rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule（4.0、2.0、1.0g/kg）, then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the od numerical value was detected.

(1)抗炎实验：将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只，补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg)，对照组给予蒸馏水，采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高，测定光密度值；(2)镇痛实验：40只小鼠分组及给药同抗炎实验，采用腹腔注射醋酸致小鼠出现扭体反应，纪录扭体次数并计算镇痛百分率；(3)抗前列腺炎实验：将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组，各10只。

Methods The bile duct of rabbit was excided and a pipe supporting bile duct was inserted through duodenal major papilla to establish a model. V.G staining and TGF-beta 1 immunohistochemistry staining on tubal wall of rabbit bile duct in different times were used to determine quantitatively the intensity of light TGF-beta 1 positive area and the relationship between the integral-optical density of bile duct tissue collagen content and time.

切开兔胆管并自十二指肠乳头行胆管内支撑方法建立模型，将不同时期梗阻段胆管壁进行V.G染色和β1型转化生长因子（transforming growth factor beta 1，TGF-β1）免疫组化染色，测定TGF-β1阳性区域的光度以及胆管组织胶原含量积分光密度随时间变化关系。

Image analysis system was applied to measure the expression level of Rb at different stages of hemangiomas and in normal skin tissues.

利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织Rb表达的积分光密度和面积。

The results show the Boer goat oocyte and pre-implantation embryo are telomerase positive. According to the densitometry of bands under the CCD imagine system, the total telomere repeats product generated of oocytes, 4-cell, 8-cell, morulae and blastocyst are 25.348, 273.832, 56.117, 251.118, 519.46, respectively.

结果表明波尔山羊卵母细胞和早期胚胎都是端粒酶阳性；依据电泳条带在凝胶成像系统下的光密度计算相对总产品产量定量比较发现，卵母细胞最低为25.348，4-细胞为273.832，至8-细胞又降低到56.117，随后快速升高，桑椹胚为251.111，囊胚端粒酶活性最高为519.46。

Methods: The immunohistochemical expression of IGFBP-2 protein was measured by image analysis in 35 surgically resected specimens of gastric cancer (include normal mucosae, primary tumors and metastases of lymph node).

对35例外科切除胃癌标本的正常粘膜、肿瘤原发灶和淋巴转移灶进行IGFBP-2蛋白的免疫组化染色，并用计算机图象分析软件测定其积分光密度。

Black clay soil ; humus ; black humic acid ; optical density index

黑粘土；腐殖质；黑色胡敏酸；光密度指数

Motic Images Analytic Technology was used to measure the IOD of calmodulin in the different stages.

采用计算机图像分析技术对不同时段分化神经元中钙调蛋白的平均积分光密度进行定量测定。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。