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Results The positive expression of AQP1 was seen in the capillary endotheliocyte of the interstitium and around bronchi and in the alveolar endothelial cells.

结果 免疫组化显示溺死时肺间质的毛细血管内皮、支气管上皮及肺泡上皮 AQP1的阳性表达,积分光密度值差别有统计学意义。

Immunocytochemistry method was used to demonstrate the expression of glial fibrillary acidic protein.

用塑料吸头划刮培养皿,制造机械损伤的模型,继续培养至72 h,分别取出不同时间点的细胞进行胶质细胞增生情况的观察和免疫组织化学染色,对胶质原纤维酸性蛋白表达产物光密度进行图像分析。

METHODS: The authors measured MPOD with heterochromatic flicker photometry, serum lutein and serum zeaxanthin by high performance liquid chromatography, and central foveal retinal thickness by optical coherence tomography in 176 patients (age range, 18-68 years) with typical forms of retinitis pigmentosa; 37 (21%) of these patients had cystoid macular edema by OCT.

作者对176例(年龄范围18-68岁)典型视网膜色素变性患者进行以下测量:采用异色闪烁光度法测量黄斑色素光密度,高效液相色谱法测量血清脂色素和血清玉米黄质,和光学相干断层扫描法测量中心凹中央视网膜厚度;其中37例(21%)患者OCT检查有黄斑囊样水肿。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富,软骨基质染色均匀,软骨细胞数目最多,Ⅱ型胶原蛋白表达最活跃,平均积分光密度值最高;青少年组软骨膜内血管减少,软骨基质染色出现明显的不均质状,软骨陷窝体积变大,并呈分隔状,陷窝内软骨细胞数目减少,II型胶原蛋白表达较儿童组减弱;成人组软骨膜血管、细胞成分明显减少,软骨膜内的纤维成分明显玻璃样变,钙盐沉积较青少年组时明显增多,Ⅱ型胶原蛋白表达较青少年组减弱。

Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5cm above the dome of acetabulum, which were fixed with ATMF'S or steel plate respectively. The animals were sacrificed and specimens were procured at 2, 4, 6, 8 and 12 weeks after operation. Samples from the fracture gaps were investigated by histology of HE and Masson staining, image pattern analysis of new bone formation.

方法] 选用10只成年杂种家犬,双侧髋臼臼顶上方1.5cm处横形截骨,分别采用ATMFS(骨盆髋臼三维记忆内固定系统)前柱固定器和5孔重建钢板内固定,于术后2、4、6、8、12周处死动物取材,行HE染色、Masson三色法染色组织学检查,图像分析新骨形成积分光密度值,研究不同应力作用模式下的骨盆骨折愈合情况。

Method]Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum, which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.Samples from the fracture gaps were investigated by histology of HE and Masson staining,image pattern analysis of new bone formation.

方法]选用10只成年杂种家犬,双侧髋臼臼顶上方1.5 cm处横形截骨,分别采用ATMFS(骨盆髋臼三维记忆内固定系统)前柱固定器和5孔重建钢板内固定,于术后2、4、6、8、12周处死动物取材,行HE染色、Masson三色法染色组织学检查,图像分析新骨形成积分光密度值,研究不同应力作用模式下的骨盆骨折愈合情况。

Method]Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum, which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.

]选用10只成年杂种家犬,双侧髋臼臼顶上方1.5 cm处横形截骨,分别采用ATMFS(骨盆髋臼三维记忆内固定系统)前柱固定器和5孔重建钢板内固定,于术后2、4、6、8、12周处死动物取材,行HE染色、Masson三色法染色组织学检查,图像分析新骨形成积分光密度值,研究不同应力作用模式下的骨盆骨折愈合情况。

[Objective]To explore the effect of continuous physiological osteogenic stress on the bone healing of canine iliopectineal crest.[Method]Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum,which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.Samples from the fracture gaps were investigated by histology of HE and Masson staining,image pattern analysis of new bone formation.

探讨持续顺应生理力线的压应力对犬骨盆弓状线部骨折愈合的影响[方法]选用10只成年杂家犬,双侧髋臼臼顶上方1.5 cm处横形截骨,分别采用ATMFS(骨盆髋臼三维记忆内固定系统)柱固定器和5孔重建钢板内固定,于术后2、4、6、8、12周处死动物取材,行HE染色、Masson三色法染色组织学检查,图像分析新骨形成积分光密度值,研究不同应力作用模式下的骨盆骨折愈合情况。

According to the extraction of cells figures, based on the edge detection the paper extracts 9 measurement parameters such as centroid, area, area rate of karyon and cytoplasm, optic density, cells and karyon shape factor according to the morphological knowledge and the medical knowledge of pathology expert.

在细胞特征提取方面,在边缘检测的基础上依据形态学知识和病理学专家的医学知识提取了诸如周长、面积、核质比、光密度、细胞及细胞核形状因子等9个度量参数。

The expression of PTEN protein was detected massively or lamellarly only innuclei. IOD represents the expression of it when the expression PTEN and MGMTprotein was investigated by confocal laser scanning microscope.

在激光共聚焦显微镜观察PTEN和MGMT蛋白在食管鳞癌组织中的表达时,用积分光密度值表示PTEN和MGMT蛋白表达的量。

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