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Methods The AGS cellseeded coverslips were prepared, then the two different status of Lactobacillus acidophilus and two different forms of Hp were added in different order. 2 hours later, the bacteria were Garm's stained, and the adherence of Hp to AGS cell were observed by microscope and the adherence index was calculated.

制备人胃上皮腺癌细胞系细胞爬片,将两种状态嗜酸乳杆菌和不同形态Hp菌分组按不同顺序加入,作用2 h,革兰染色后光镜观察分析Hp黏附指数产生的变化,荧光抗体染色法定性评价黏附于AGS细胞的Hp密度的改变。

In this paper, we optimized the thickness of the doublers and triplers, mismatch angles and other parameters of Ⅰ/Ⅱ angle detuning and Ⅱ/Ⅱ polarization mismatch third harmonic generation schemes by numerical calculation way.

采用四阶龙格-库塔数值计算方法,以Ⅰ/Ⅱ角度失谐和Ⅱ/Ⅱ偏振失配两种三倍频方案为例,给出了入射基频光为平面方波,功率密度5GW/cm2的情况下,KDP晶体三倍频参数的优化结果。

Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括:[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术;[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离,测定了不同顺式异构体的吸光系数,建立了天然番茄红素几何异构体组成的检测方法,为区分和鉴定人工合成品和天然产物奠定了基础;[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值,并建立了超临界CO_2萃取的实验室方法;[4]探索了提高番茄红素超临界流体萃取效率的方法,证明了物料前处理对提高萃取效率起了极其重要的作用;[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况,发现了大量顺式异构体的存在,并证实了几何异构体组成在血清中发生了显著变化;[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律;[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力;[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能;[9]首次发现大鼠前列腺内存在番茄红素C31降解片段,并证实其仍具有淬灭单线态氧的能力。

CVA was used to study the relationship between 6 cutting heights of 3, 6, 10, 14, 18 and 24cm, and 3 population traits of herbage yield, leaf area and tiller density, on manned swards of tall rescue grown under constant light and temperature regimes. The first canonical variate, which is very significant, explained 98%of separation among the heights.

在控制光温的人工草地上,高羊茅草丛群体的3个特征-牧草产量、叶面积和分蘖密度与刈割高度(3、6、10、14、18和24cm)的关系研究中采用CVA,结果表明,第一典型相关极显著,且第一典型变量能反映刈割高度间98%的变异。

In this paper, the structure and its morphology of the as-spun isotactic polypro- pylene monofilaments has been investigated by using cross polarized microscope, SALS, SEM, WAXD, density, birefringence and Hardy's thin cross-section device.

本文采用正交偏振光、小角激光散射、扫描电子显微镜,广角X光衍射、密度、双折射和切片技术,对工业用丝——IPP单丝的未拉伸丝的结构及其形态进行了广泛的研究。

Diffraction limit ; near field optics ; Super RENS ; super high density optical data storage ; near field photolithography ; namometer photonics transistor ; nonlinear optical device

衍射极限;近场光学;超分辨率近场结构;超高密度光学数据存储;近场光刻;纳米光子学晶体管;非线性光学器件

A black hole is a point in space that is so dense with matter that its gravitational field will not let anything - not even light - escape.

黑洞就是太空中的一个点,此点密度甚高,使得任何物质甚至光都无法脱离它的引力。

Can reverse JA-induced migration inhibition JA-treated cells also showed significantly higher adhesion to laminin In conclusion these results indicate a novel anti-tumor role of JA via reducing reorganization of cytoskeleton increasing integrin 3 expression and adhesion ability to inhibit tumor cells migration Furthermore immunofluorescence staining of phalloidin/actin indicated that JA-treated cells exhibited cytoskeleton rearrangement and significant decreases in the number and length of filopodia Activity of Rho-family small GTPases was detected by a GST-pull down assay and showed that GTP-loaded Cdc42 and Rac were decreased in JA-treated cells In the future we hope these findings will be helpful to further understand the mechanisms underlying tumor progression in oral cancer and subsequently improve the cancer prevention and treatment

以抗体阻断integrin ?3可以有效使OC2恢复被JA所抑制的迁移能力。细胞贴附实验指出JA能提高OC-2在laminin环境下的贴附能力。当我们进一步以免疫萤光染色观察细胞,发现在JA处理下的OC-2,其外形、细胞骨架和细胞边缘的filopodia的密度和长度明显减少,利用GST-pull down assay侦测到Cdc42-GTP和Rac-GTP的表现量降低。推论爵床素A可能透过刺激口腔癌细胞大量表现integrin ?3,使细胞贴附能力增加或是透过改变细胞骨架来降低细胞迁移能力。希望本研究对於JA所做的抗癌及抗转移效应评估能提供口腔癌治疗在研发上一些帮助。

Focusing on all these functional traits, 13 functional groups were identified by TWINSPAN (two-way indicator species analysis), and their change with different environmental factors, disturbance types and recovery time was ordinated by DCCA(detrended canonical correspondence analysis).

本文以海南岛霸王岭林区作为海南岛热带林的典型区域,以反映不同环境因子、干扰类型和恢复时间的热带山地雨林为研究对象,在大面积(15hm~2)样地调查的基础上,依据物种的7个功能特性(喜光性、潜在高度、板根大小、木材密度、种子生物量、传播方式和落叶/常绿性),分别把物种划分为不同类别的功能群,应用NMS分析了各功能群随不同干扰类型和恢复时间热带山地雨林群落的变化规律。

The C-V profiles display two well-separated sharp peaks that are originated from the ground and excited states of the QDs, respectively , suggesting an improved uniformity and decreased dot density. The addition of Sb can retard the 3D growth mode and expand the critical thickness of the growth mode from 2D to 3D.

G。比值从0.6937提升至1.2102与光性分析有相同趋势,半高宽有下降趋势显示随Sb增加量子点有较均匀的现象;在Sb含量最多的样品发现,随著量测频率下降基态的浓度有明显增加的趋势(2×1017增加至2.15×1019),使的基态峰值显得更陡峭,由於可以在电性明显的量测出基态的变化,是因为量子点密度低与均匀度好的原因,这个结果与之前的分析有相同趋势。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。