先约

Approximately 1% of mutations occur de novo ; thus, 1% of probands have only one parent who is heterozygous.

约1%的突变是新生突变；因此，1%的先证者只有一个杂合子的父母。

During the titration of Ca〓CaM by La〓, the hydrid complex La〓Ca〓CaM forms as the intermediate species. La〓 can bind to calmodulin with high affinity (K〓=10nmol/L) and obvious cooperation between the two global domains. The secondary structure of La〓CaM and Ca〓CaM are highly similar, but there are obvious differences in ternary structures. Excessive La〓 changes the conformation of calmodulin greatly.

实验结果表明，La〓在钙调蛋白上的结合位点与钙离子相同；在结合顺序上La〓以较小的优势先结合在钙调蛋白的N末端；La〓Ca〓CaM是La〓与含钙的钙调蛋白作用时的主要产物；La〓强烈地与钙调蛋白结合，其解离常数约10nmol/L，各个结合位点之间存在明显的协同效应；La〓CaM与Ca〓CaM在二级结构上高度相似，但是在高级结构上有明显差异。

If you don't mind, Johnathan, while you and Mr.

如果你不介意，约翰逊，你和孙先生先聊着，我看看会议安排的如何。

Umbrella form inflorescence normally 2~4 bunch gives birth to short branch of Yu Xie armpit, short branch grows 1~2 millimeter; Inflorescence total stalk grows 5~10 millimeter, by short fluff; Bud piece 4, wide egg form, have 5~6 arteries and veins, outside a few without wool, inside face by white short fluff; Inflorescence of each umbrella form has beautiful 4~6, the leaf is opened or open at the same time with the leaf first; Peduncular grow about 1.5 millimeter, close by white fluff; Perianth piece 6, elliptic, two sides by short fluff; In male flower can Yo is staminal 9, filament has grey yellow fluff, form of heart of shape of aegis of body of gland of ministry of the 3rd round of stamen radical, do not have a handle nearly, degrade pistil is not had; Stamen degrades in female flower 9, staff state, by grey yellow fluff, ovarian crock form, style is dumpy, post head shape, shallow crack.

伞形花序通常2~4个簇生于叶腋短枝上，短枝长1~2毫米；花序总梗长5~10毫米，被短柔毛；苞片4，宽卵形，有5~6条脉，外面几无毛，内面被白色短柔毛；每一伞形花序有花4~6朵，先叶开放或与叶同时开放；花梗长约1.5毫米，密被白色柔毛；花被片6，椭圆形，两面被短柔毛；雄花中能育雄蕊9，花丝有灰黄色柔毛，第三轮雄蕊基部腺体盾状心形，近无柄，退化雌蕊无；雌花中退化雄蕊9，棍棒状，被灰黄色柔毛，子房壶形，花柱粗短，柱头头状，具浅裂。

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

Leaves toward base of stem: petiole ca. 0.8-1.3 cm, tomentose, prophyll up to 1/2 as long as petiole; leaf blade ovate or narrowly ovate, 10-18 × 5-10 cm, papery, glabrous to abaxially tomentose, adaxially pilose along veins, base cordate, oblique, apex acute or acute-acuminate; veins 9, apical pair arising 1-3 cm above base, others basal; reticulate veins conspicuous.

约的对于scircapetiole的基部的叶0.8-1.3厘米，被绒毛，先出叶可达1/2的倍于叶柄；叶片卵形或狭卵形， 10-18 * 5-10 厘米，纸质，无毛到背面的被绒毛，正面具柔毛的沿脉，基部心形，偏斜，先端锐尖的或锐尖尖；脉9，出现的顶端的对超过基部1-3厘米，其它人基部；网脉明显穗状花序对生。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

Mr John so has battened down for any challenge from his opponents.

约翰逊生先已做好充分准备迎接反对派的挑战。

The purpose of this study was to examine the difference between using bladder scan and catheterization on 87 rehabilitation patients to evaluation the amount of residual urine. After voiding, each subject was scanned with a BladderScan BVI 3000, then catheterized for postvoid residual urine volume. Repeat measure ANOVA analysis showed that the factors of gender, diagnosis, posture, the thickness of abdominal fat, bladder shape, the amount of urine, and the interval of operation time have no difference in this study. It takes 45 ± 18 seconds (range: 17-119) to accomplish a bladder scan, and it takes 280 ±106 seconds (range: 136-664) for nursing staff to complete a catheterization for patient. However, the catheterization process takes nursing staff 3-8 times longer then operating a bladder scan. This would diminish unnecessary catheterization and save on medical staff resources.

本研究对87位需做余尿量评估的复健病人，在排尿后以膀胱超音波BVI 3000机型测量扫描余尿量，然后接著给予间歇导尿，以repeat measures ANOVA分析，结果发现膀胱超音波及导尿测量所得尿量是无差异(F=0.38 p=。68)，再以性别、诊断、姿势、腹部脂肪厚度、膀胱形状、尿量、操作间隔时间等因素做分析，以repeat measures ANOVA检定亦无差异，同时测量两种测量方法所花费护理时数：膀胱超音波平均为45±18秒(range： 17-119秒)，导尿为平均280±106秒(range： 136-664秒)，结论是膀胱超音波与导尿方式对余尿量的测量一样好，膀胱超音波与导尿时间相差约3-8倍的护理时数，先以超音波来测量余尿量可以减少不必要的导尿次数及节省医疗人力成本。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。